Protein Info for GFF5906 in Variovorax sp. SCN45
Annotation: Nitrilase (EC 3.5.5.7)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 56% identical to NITR3_UNKP: Nitrilase 3 (BD5308) from Unknown prokaryotic organism
KEGG orthology group: K01502, aliphatic nitrilase [EC: 3.5.5.7] (inferred from 92% identity to vpe:Varpa_0597)Predicted SEED Role
"Nitrilase (EC 3.5.5.7)" (EC 3.5.5.7)
MetaCyc Pathways
- superpathway of acrylonitrile degradation (3/3 steps found)
- acrylonitrile degradation II (1/1 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 3.5.5.7
Use Curated BLAST to search for 3.5.5.7
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (347 amino acids)
>GFF5906 Nitrilase (EC 3.5.5.7) (Variovorax sp. SCN45) MASTPTTVRAAAIQIAPDFERPEGTLERVCGAIDAAAARGAQLAVFPETFVPYYPYFSFV LPPVLQGAPHLRLAERAVVVPGPVTQAVAERARARNMVVVLGVNERDHGSLYNTQLVFDA DGTLALKRRKITPTYHERMVWGMGDGAGLKVVDTAVGRVGALACWEHYNPLARYALMAQH EQIHCAQFPGSLVGPIFAEQMEVTIRHHALESGCFVVNATGWLTDEQIRSVTPDANLQKA LRGGCHTAIVSPEGKHLAPPLTEGEGMVVADLDMALIAKRKRMMDSVGHYARPELLSLAI NDRPAQTTVPMHLTQPFASRSPEHDHDDTAAQPAIDDRAPVLRVAAG