Protein Info for GFF59 in Sphingobium sp. HT1-2

Annotation: Glycyl-tRNA synthetase beta chain (EC 6.1.1.14)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 734 TIGR00211: glycine--tRNA ligase, beta subunit" amino acids 2 to 511 (510 residues), 479.3 bits, see alignment E=1.5e-147 PF02092: tRNA_synt_2f" amino acids 4 to 593 (590 residues), 620.8 bits, see alignment E=2.7e-190 PF05746: DALR_1" amino acids 625 to 726 (102 residues), 39.9 bits, see alignment E=4e-14

Best Hits

Predicted SEED Role

"Glycyl-tRNA synthetase beta chain (EC 6.1.1.14)" (EC 6.1.1.14)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.14

Use Curated BLAST to search for 6.1.1.14

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (734 amino acids)

>GFF59 Glycyl-tRNA synthetase beta chain (EC 6.1.1.14) (Sphingobium sp. HT1-2)
MTDFLLELRCEEIPARMQLKASDDLARLFTEELAKAGLKPALIDSFVTPRRLALIARDLP
LETAAVSEEFKGPRTSAPAQALEGFLRKTGLTQDQLEDRDGVWFAVVNKPGRATTDVLAE
AVPAVIRAFPWPKSMRWGAASATTESLRWVRPLQGIIALLGGEIVPLSVEGIEAGRETVG
HRFHSTGAIRIDGADDYADKLRAAYVIVSHQERQAIIEAKAGEAAAAKGYSVIEDKGLVA
ENAGLTEWPVPLLGDFDPEFLEVPPEVIQLTLRINQKYFVLRDAAGKLAPAFICTANIEA
KDGGVAIVAGNRKVLAARLSDARFFWQQDRKTPLAEQAQKLGRITFHEKLGTVADKVERV
AKLAEWLASEGIVPNCDPVLARQAAELCKADLVTEMVGEFPELQGVMGGYYARAEGLPDA
VADAIRDHYKPVGQGDDVPTAPVTVAVSLADKLDSLAGFFLIGETPTGSRDPFALRRSAL
GLISLVQEAGLRLGLEKLISRQILWLDESRVRSLINDVSSLVDGAIDLFDGDVPDEFIDS
AYEKSKSSRDDKLQKMIDNAIQARTKLLDFFADRLKVQQKEAGVRHDLIDAVFALGGEDD
LVRLLARVKALQSFVATDDGANLLAGYKRAANILKKEGVEQAEAISLSYEPEKAEADLIA
ALDAAEPQAAQAVAAEEFEGAMAALATLRAPIDAFFETVTVNDADPAKRTTRLALLARVR
DAVHNVADFSKITG