Protein Info for GFF5845 in Variovorax sp. SCN45

Annotation: NAD synthetase (EC 6.3.1.5)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00552: NAD+ synthetase
  • amino acids 28 to 276 (249 residues), 207 bits, E=1.4e-65
• PF02540: NAD_synthase
  • amino acids 31 to 275 (245 residues), 201.9 bits, E=4.5e-64

Best Hits

Swiss-Prot: 67% identical to NADE_STRCO: NH(3)-dependent NAD(+) synthetase (nadE) from Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)

KEGG orthology group: K01916, NAD+ synthase [EC: 6.3.1.5] (inferred from 93% identity to vpe:Varpa_0737)

MetaCyc: 50% identical to NH3-dependent NAD+ synthetase (Escherichia coli K-12 substr. MG1655)
NAD(+) synthase. [EC: 6.3.1.5]

Predicted SEED Role

"NAD synthetase (EC 6.3.1.5)" in subsystem NAD and NADP cofactor biosynthesis global or NAD regulation (EC 6.3.1.5)

MetaCyc Pathways

• aspartate superpathway (24/25 steps found)
• NAD de novo biosynthesis I (6/6 steps found)
• NAD salvage pathway II (PNC IV cycle) (5/5 steps found)
• NAD salvage pathway I (PNC VI cycle) (6/7 steps found)
• NAD de novo biosynthesis III (5/6 steps found)
• NAD de novo biosynthesis IV (anaerobic) (5/6 steps found)

KEGG Metabolic Maps

• Nicotinate and nicotinamide metabolism
• Nitrogen metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.3.1.5

Use Curated BLAST to search for 6.3.1.5

Protein Sequence (285 amino acids)

>GFF5845 NAD synthetase (EC 6.3.1.5) (Variovorax sp. SCN45)
MSADLTPVDDTQREIIAALHVAPVFDAASELERRVDFLASYLKQTGLKTLVLGISGGVDS
LTAGCLAQRAVEKLRAEGRDASFIAMRLPYGVQKDEAEAQASLAVIRPDRTITVDIRPAA
DGMLAALKAGDLKFRDAAHEDFVLGNIKARERMVAQFAVAGAHDGIVIGTDHAAEALMGF
FTKFGDGAADITPLTGLNKRRVRAVAQLLGAPDALVYKVPTADLESLVPGKPDEDAFGVT
YEQIDDFLEGKPVSAEARRIILSTHRKSAHKRALPVEPPAPAALG