Protein Info for GFF584 in Sphingobium sp. HT1-2

Annotation: 3-mercaptopyruvate sulfurtransferase (EC 2.8.1.2)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00581: Rhodanese
  • amino acids 6 to 126 (121 residues), 46.9 bits, E=1.6e-16
  • amino acids 156 to 268 (113 residues), 53.7 bits, E=1.2e-18

Best Hits

Swiss-Prot: 42% identical to THTM_RAT: 3-mercaptopyruvate sulfurtransferase (Mpst) from Rattus norvegicus

KEGG orthology group: K01011, thiosulfate/3-mercaptopyruvate sulfurtransferase [EC: 2.8.1.1 2.8.1.2] (inferred from 77% identity to sjp:SJA_C1-06900)

MetaCyc: 42% identical to 3-mercaptopyruvate sulfurtransferase (Rattus norvegicus)
3-mercaptopyruvate sulfurtransferase. [EC: 2.8.1.2]

Predicted SEED Role

"Thiosulfate sulfurtransferase, rhodanese (EC 2.8.1.1)" (EC 2.8.1.1)

MetaCyc Pathways

• thiosulfate disproportionation IV (rhodanese) (1/1 steps found)
• L-cysteine degradation III (1/2 steps found)
• sulfide oxidation IV (mitochondria) (2/5 steps found)

KEGG Metabolic Maps

• Cysteine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.8.1.1 or 2.8.1.2

Protein Sequence (282 amino acids)

>GFF584 3-mercaptopyruvate sulfurtransferase (EC 2.8.1.2) (Sphingobium sp. HT1-2)
MDIIVSTDWLAGEIGKPDLVILDASLFLPGTPRDPRAEFDAAHIPGAAFMDLPSLSDPAD
PVPGQLPPDALMTQRTQALGVNADSRIVVYDNSPTHSAARGWWMMRVYGLGASAAILDGG
LPKWVAEGRQVDSGTPAMAPGNAVARLDRTQVRSKADILANLASGTAQLLDARGAGRFTG
AEAEPRPGMASGHIPGSRNIPSSAFFAADNSLKQGEELRRLLLDAGTDLDRPIITTCGSG
VTAAIILAALETLGKRDVSLYDGSWSEWGFDPATPKATGPAA