Protein Info for GFF5837 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: Biotin carboxylase of acetyl-CoA carboxylase (EC 6.3.4.14)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00289: Biotin_carb_N
  • amino acids 1 to 44 (44 residues), 55.2 bits, 2.3e-18
• PF02786: CPSase_L_D2
  • amino acids 50 to 256 (207 residues), 243 bits, E=6.3e-76
• PF07478: Dala_Dala_lig_C
  • amino acids 79 to 225 (147 residues), 24.3 bits, E=5.1e-09
• PF02785: Biotin_carb_C
  • amino acids 270 to 377 (108 residues), 133.8 bits, E=6.3e-43

Best Hits

Swiss-Prot: 54% identical to ACCC1_BACSU: Biotin carboxylase 1 (accC1) from Bacillus subtilis (strain 168)

KEGG orthology group: K01961, acetyl-CoA carboxylase, biotin carboxylase subunit [EC: 6.3.4.14 6.4.1.2] (inferred from 58% identity to rmr:Rmar_0675)

MetaCyc: 47% identical to 4-hydroxyacetophenone carboxylase biotin carboxylase subunit (Aromatoleum aromaticum EbN1)
RXN-9833

Predicted SEED Role

"Biotin carboxylase of acetyl-CoA carboxylase (EC 6.3.4.14)" in subsystem Fatty Acid Biosynthesis FASII (EC 6.3.4.14)

MetaCyc Pathways

• biotin-carboxyl carrier protein assembly (4/4 steps found)
• 4-ethylphenol degradation (anaerobic) (4/6 steps found)
• fatty acid biosynthesis initiation (type I) (1/3 steps found)
• 3-hydroxypropanoate cycle (8/13 steps found)
• 3-hydroxypropanoate/4-hydroxybutanate cycle (11/18 steps found)
• glyoxylate assimilation (6/13 steps found)
• jadomycin biosynthesis (3/9 steps found)
• superpathway of the 3-hydroxypropanoate cycle (9/18 steps found)
• superpathway of mycolate biosynthesis (27/239 steps found)

KEGG Metabolic Maps

• Fatty acid biosynthesis
• Propanoate metabolism
• Pyruvate metabolism
• Tetracycline biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 6.3.4.14, 6.4.1.2

Use Curated BLAST to search for 6.3.4.14 or 6.4.1.2

Protein Sequence (393 amino acids)

>GFF5837 Biotin carboxylase of acetyl-CoA carboxylase (EC 6.3.4.14) (Hydrogenophaga sp. GW460-11-11-14-LB1)
VAAAKGTGCDAIHPGYGFLSERASFQRLCAEQGLVFVGPDADAIERMGNKINAIVAARAA
GVPVLPGRERIGNVAELLTCAGEIGYPVLIKASAGGGGRGMRLLHRPQDAEAAFASAQAE
ALAAFGDGTVYLEKYIQDARHIEIQVIADAFGRCCHLFERDCSVQRRHQKLVEESPSPVL
SADMRQAMAKAAVDLTLAVGYRNAGTVEFVFDKASGAFYFLEMNTRVQVEHPVTEAVTGI
DIVREQIRIAMGLPLSFVQEDIRMDGHAIECRVNAEDPHDGFRPSPGRVREWLPPDHPWV
RLDTHVYPGYLVPPFYDSMVAKVIAHGPTREYAIAQMLAYLRAFRVTGITTTIPLQIQVM
EHPDFAAGDVTTTWLEQTILPSLNKKNTEAPHA