Protein Info for GFF5835 in Variovorax sp. SCN45

Annotation: Uroporphyrinogen III decarboxylase (EC 4.1.1.37)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 92% identical to DCUP_VARPS: Uroporphyrinogen decarboxylase (hemE) from Variovorax paradoxus (strain S110)

KEGG orthology group: K01599, uroporphyrinogen decarboxylase [EC: 4.1.1.37] (inferred from 92% identity to vpe:Varpa_0746)

MetaCyc: 60% identical to uroporphyrinogen decarboxylase (Escherichia coli K-12 substr. MG1655)
Uroporphyrinogen decarboxylase. [EC: 4.1.1.37]

Predicted SEED Role

"Uroporphyrinogen III decarboxylase (EC 4.1.1.37)" in subsystem Experimental tye or Heme and Siroheme Biosynthesis (EC 4.1.1.37)

MetaCyc Pathways

superpathway of heme b biosynthesis from glutamate (9/10 steps found)
superpathway of heme b biosynthesis from glycine (6/8 steps found)
heme b biosynthesis I (aerobic) (3/4 steps found)
heme b biosynthesis II (oxygen-independent) (3/4 steps found)
heme b biosynthesis V (aerobic) (3/4 steps found)
superpathway of heme b biosynthesis from uroporphyrinogen-III (4/6 steps found)
heme b biosynthesis IV (Gram-positive bacteria) (1/5 steps found)
3,8-divinyl-chlorophyllide a biosynthesis I (aerobic, light-dependent) (3/9 steps found)
3,8-divinyl-chlorophyllide a biosynthesis II (anaerobic) (3/9 steps found)
3,8-divinyl-chlorophyllide a biosynthesis III (aerobic, light independent) (3/9 steps found)
superpathway of bacteriochlorophyll a biosynthesis (6/26 steps found)

KEGG Metabolic Maps

Porphyrin and chlorophyll metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.1.1.37

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (369 amino acids)

>GFF5835 Uroporphyrinogen III decarboxylase (EC 4.1.1.37) (Variovorax sp. SCN45)
MPFAPLQNDTFLRACWRQATDHTPVWLMRQAGRYLPEYVATRAKAGSFMGLATNTGYATE
VTLQPLERYALDAAILFSDILTVPDAMGLGLSFEAGEGPRFARPVRDEAAVAALEVPDMQ
KLRYVFDAVASIRKALNGRVPLIGFSGSPWTLACYMVEGAGSSDYRLVKSMLYSRPDLMH
RILAVNAESVAAYLNAQIDAGAQAVMVFDSWGGVLADGAFQEFSLAYTARVLAGLKRTGA
DGQPVPRIVFTKGGGLWLDAMRELDCQVLGVDWTVNLASARKQVAEGTDGKAKALQGNID
PNVLFAPPDRIEAEVAKVLDAFGKPHADRATQGPTHIFNLGHGISQFTPPEHVAALVQAV
HARSRALRG