Protein Info for PS417_02965 in Pseudomonas simiae WCS417

Annotation: acetyl-CoA carboxylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00289: Biotin_carb_N
  • amino acids 8 to 116 (109 residues), 149.7 bits, E=1e-47
• TIGR00514: acetyl-CoA carboxylase, biotin carboxylase subunit
  • amino acids 8 to 451 (444 residues), 725.4 bits, E=1.3e-222
• PF02786: CPSase_L_D2
  • amino acids 121 to 328 (208 residues), 269.3 bits, E=5.6e-84
• PF02222: ATP-grasp
  • amino acids 147 to 299 (153 residues), 29.4 bits, E=1.5e-10
• PF07478: Dala_Dala_lig_C
  • amino acids 148 to 297 (150 residues), 30.6 bits, E=6e-11
• PF02785: Biotin_carb_C
  • amino acids 342 to 447 (106 residues), 132.3 bits, E=1.9e-42

Best Hits

Swiss-Prot: 84% identical to ACCC_PSEAE: Biotin carboxylase (accC) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K01961, acetyl-CoA carboxylase, biotin carboxylase subunit [EC: 6.3.4.14 6.4.1.2] (inferred from 99% identity to pfs:PFLU0617)

MetaCyc: 67% identical to biotin carboxylase (Escherichia coli K-12 substr. MG1655)
Biotin carboxylase. [EC: 6.3.4.14]

Predicted SEED Role

"Biotin carboxylase of acetyl-CoA carboxylase (EC 6.3.4.14)" in subsystem Fatty Acid Biosynthesis FASII (EC 6.3.4.14)

MetaCyc Pathways

• biotin-carboxyl carrier protein assembly (4/4 steps found)
• fatty acid biosynthesis initiation (type I) (1/3 steps found)
• 3-hydroxypropanoate cycle (6/13 steps found)
• jadomycin biosynthesis (3/9 steps found)
• 3-hydroxypropanoate/4-hydroxybutanate cycle (9/18 steps found)
• glyoxylate assimilation (4/13 steps found)
• superpathway of the 3-hydroxypropanoate cycle (6/18 steps found)
• superpathway of mycolate biosynthesis (21/239 steps found)

KEGG Metabolic Maps

• Fatty acid biosynthesis
• Propanoate metabolism
• Pyruvate metabolism
• Tetracycline biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 6.3.4.14, 6.4.1.2

Use Curated BLAST to search for 6.3.4.14 or 6.4.1.2

Sequence Analysis Tools

See A0A1N7TZ75 at UniProt or InterPro

Protein Sequence (453 amino acids)

>PS417_02965 acetyl-CoA carboxylase (Pseudomonas simiae WCS417)
MLKPAKKLQKVLIANRGEIALRILRACKEEGIKTVAVYSTADTELMHVKLADESICIGPP
LATNSYLKVSNIIAAAEVTGADGIHPGYGFLAENADFAEQVEKSGFAFIGPKAETIRLMG
DKVSAKDAMIAAGVPTVPGSDGPLPEDEETALRIGREVGYPVIIKAAGGGGGRGMRVVHK
EEDLIEAAKQTRSEAGAWFGNPMVYLEKYLTNPRHVEVQVLSDGQGHAIHLGDRDCSLQR
RHQKVLEEAPAPGLDEKARQEVLARCVKACIDINYRGAGTFEFLYENGRFYFIEMNTRVQ
VEHPVSEMVTGIDIVKEMLSIAAGNPLSFTQDDVKMHGHSLECRINAEDPKTFIPSPGLV
KHFHAPGGNGVRVDSHLYSGYKVPSNYDSLIGKLITWGATRDEAMARMRNALDEIVVDGI
KTNIPLHRDLVRDEGFCEGGVNIHYLEHKLANQ