Protein Info for GFF582 in Sphingobium sp. HT1-2

Annotation: NADPH-dependent 7-cyano-7-deazaguanine reductase (EC 1.7.1.13)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 81% identical to QUEF_NOVAD: NADPH-dependent 7-cyano-7-deazaguanine reductase (queF) from Novosphingobium aromaticivorans (strain ATCC 700278 / DSM 12444 / CIP 105152 / NBRC 16084 / F199)

KEGG orthology group: K09457, 7-cyano-7-deazaguanine reductase [EC: 1.7.1.13] (inferred from 92% identity to sjp:SJA_C1-06920)

Predicted SEED Role

"NADPH dependent preQ0 reductase (EC 1.7.1.13)" (EC 1.7.1.13)

MetaCyc Pathways

queuosine biosynthesis I (de novo) (3/4 steps found)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.7.1.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (150 amino acids)

>GFF582 NADPH-dependent 7-cyano-7-deazaguanine reductase (EC 1.7.1.13) (Sphingobium sp. HT1-2)
MTDTPLHLGRASTLPQRPEDAVLDYVPNPRPGKPYLVRFTAPEFTSLCPVTGQPDFAHLV
IDYAPGATIVESKALKLFLGSFRNHAGFHEDCTVGIGERLFTEMQPIWLRIGGYWYPRGG
IPIDVFWQSGEPPAGLWLPPQDVPGYRGRG