Protein Info for PS417_02940 in Pseudomonas simiae WCS417
Annotation: phosphoribosylaminoimidazolecarboxamide formyltransferase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 98% identical to PUR9_PSEFS: Bifunctional purine biosynthesis protein PurH (purH) from Pseudomonas fluorescens (strain SBW25)
KEGG orthology group: K00602, phosphoribosylaminoimidazolecarboxamide formyltransferase / IMP cyclohydrolase [EC: 2.1.2.3 3.5.4.10] (inferred from 98% identity to pfs:PFLU0612)MetaCyc: 69% identical to bifunctional AICAR transformylase/IMP cyclohydrolase (Escherichia coli K-12 substr. MG1655)
IMP cyclohydrolase. [EC: 3.5.4.10]; Phosphoribosylaminoimidazolecarboxamide formyltransferase. [EC: 3.5.4.10, 2.1.2.3]
Predicted SEED Role
"IMP cyclohydrolase (EC 3.5.4.10) / Phosphoribosylaminoimidazolecarboxamide formyltransferase (EC 2.1.2.3)" in subsystem De Novo Purine Biosynthesis (EC 2.1.2.3, EC 3.5.4.10)
MetaCyc Pathways
- superpathway of histidine, purine, and pyrimidine biosynthesis (44/46 steps found)
- superpathway of purine nucleotides de novo biosynthesis I (21/21 steps found)
- superpathway of purine nucleotides de novo biosynthesis II (24/26 steps found)
- inosine-5'-phosphate biosynthesis I (6/6 steps found)
- inosine-5'-phosphate biosynthesis II (5/5 steps found)
- inosine-5'-phosphate biosynthesis III (5/6 steps found)
KEGG Metabolic Maps
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of plant hormones
- One carbon pool by folate
- Purine metabolism
Isozymes
No predicted isozymesUse Curated BLAST to search for 2.1.2.3 or 3.5.4.10
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A1N7TXG0 at UniProt or InterPro
Protein Sequence (535 amino acids)
>PS417_02940 phosphoribosylaminoimidazolecarboxamide formyltransferase (Pseudomonas simiae WCS417) MTDQTTRLPIRRALISVSDKTGILEFARELEALGVEILSTGGTFKLLQDNGVAAVEVADY TGFAEMMDGRVKTLHPKIHGGILGRRGTDDAIMAEHGIKPIDLVAVNLYPFEATINKPGC DLPTAIENIDIGGPTMVRSAAKNHKDVAIVVNASDYANVLESLKAGGLTYAQRFDLMLKA FEHTAAYDGMIANYMGTVDQTAETLSTSGRSQFPRTFNSQFIKAQEMRYGENPHQSAAFY VEAKPAEVGIATATQLQGKELSYNNVADTDAALECVKSFVKPACVIVKHANPCGVAVSPD AEGGIRQAYELAYATDTESAFGGIIAFNRELDAETAKAIVERQFVEVIIAPSVSEEARAI VAAKANVRLLACGEWSAERAAAWDYKRVNGGLLVQSRDIGMIGSQDLKVVTQRAPTEQEI NDLIFAWKVAKYVKSNAIVYAKNRQTIGVGAGQMSRVNSARIAAIKAEHAGLQVAGSVMA SDAFFPFRDGLDNAAKAGVTAVIQPGGSMRDAEVIAAADEAGIAMVFTGMRHFRH