Protein Info for GFF5763 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: Protein-L-isoaspartate O-methyltransferase (EC 2.1.1.77)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 231 PF01135: PCMT" amino acids 16 to 204 (189 residues), 105.7 bits, see alignment E=1e-33 PF00398: RrnaAD" amino acids 75 to 131 (57 residues), 40.3 bits, see alignment E=7.1e-14 PF05175: MTS" amino acids 79 to 147 (69 residues), 26.1 bits, see alignment E=2.1e-09 PF06325: PrmA" amino acids 84 to 185 (102 residues), 22.6 bits, see alignment E=2.3e-08 PF13847: Methyltransf_31" amino acids 87 to 172 (86 residues), 34.2 bits, see alignment E=7.2e-12 PF13649: Methyltransf_25" amino acids 91 to 171 (81 residues), 41.9 bits, see alignment E=4.9e-14 PF08241: Methyltransf_11" amino acids 92 to 170 (79 residues), 33 bits, see alignment E=2.6e-11

Best Hits

Swiss-Prot: 38% identical to PIMT2_MARHV: Protein-L-isoaspartate O-methyltransferase 2 (pcm2) from Marinobacter hydrocarbonoclasticus (strain ATCC 700491 / DSM 11845 / VT8)

KEGG orthology group: K00573, protein-L-isoaspartate(D-aspartate) O-methyltransferase [EC: 2.1.1.77] (inferred from 63% identity to pol:Bpro_1666)

Predicted SEED Role

"Protein-L-isoaspartate O-methyltransferase (EC 2.1.1.77)" in subsystem Ton and Tol transport systems (EC 2.1.1.77)

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.77

Use Curated BLAST to search for 2.1.1.77

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (231 amino acids)

>GFF5763 Protein-L-isoaspartate O-methyltransferase (EC 2.1.1.77) (Hydrogenophaga sp. GW460-11-11-14-LB1)
MTTSSALSLSQARFNMIEQQIRPWDVLDARVLDLLDRVHREQFVPAAHRSLAFADMELPL
SSPAVDGQVMLAPRVEARLVQDLMLKPTDKVLEIGAGSGHMAAMLASLAQRVVSIEIDEA
LARLARENLQKAGIGNAEVRCADAAANGFAACKDGGPFDAIVLSGSVAEIPSALLTLLAP
GGRLAAIVGFEPMMRATIVTRTGEAAFQTAQPWDTVAPRLRHFPEPSRFRF