Protein Info for GFF570 in Variovorax sp. SCN45

Annotation: 3,4-dihydroxy-2-butanone 4-phosphate synthase (EC 4.1.99.12) / GTP cyclohydrolase II (EC 3.5.4.25)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00506: 3,4-dihydroxy-2-butanone-4-phosphate synthase
  • amino acids 21 to 216 (196 residues), 239.9 bits, E=9.3e-76
• PF00926: DHBP_synthase
  • amino acids 24 to 215 (192 residues), 277.3 bits, E=5.7e-87
• PF00925: GTP_cyclohydro2
  • amino acids 229 to 381 (153 residues), 120.6 bits, E=5.2e-39

Best Hits

Swiss-Prot: 53% identical to RIBB_PSEAE: 3,4-dihydroxy-2-butanone 4-phosphate synthase (ribB) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K14652, 3,4-dihydroxy 2-butanone 4-phosphate synthase / GTP cyclohydrolase II [EC: 3.5.4.25 4.1.99.12] (inferred from 96% identity to vpe:Varpa_3524)

Predicted SEED Role

"3,4-dihydroxy-2-butanone 4-phosphate synthase (EC 4.1.99.12) / GTP cyclohydrolase II (EC 3.5.4.25)" in subsystem Molybdenum cofactor biosynthesis or Riboflavin, FMN and FAD metabolism (EC 3.5.4.25, EC 4.1.99.12)

MetaCyc Pathways

• flavin biosynthesis I (bacteria and plants) (8/9 steps found)
• flavin biosynthesis III (fungi) (7/9 steps found)
• 6-hydroxymethyl-dihydropterin diphosphate biosynthesis III (Chlamydia) (3/5 steps found)
• toxoflavin biosynthesis (3/7 steps found)
• flavin biosynthesis II (archaea) (5/10 steps found)

KEGG Metabolic Maps

• Riboflavin metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.5.4.25

Use Curated BLAST to search for 3.5.4.25 or 4.1.99.12

Protein Sequence (384 amino acids)

>GFF570 3,4-dihydroxy-2-butanone 4-phosphate synthase (EC 4.1.99.12) / GTP cyclohydrolase II (EC 3.5.4.25) (Variovorax sp. SCN45)
MNASVTPIGAPRAARAPAPISPVEDIVADLAAGRMVILVDEEDRENEGDIVIAADHITPE
AINFMARHARGLICLTLSREMCERLQLPPMVQRNGAKHSTAFTVSIEAAEGVTTGISAAD
RARTVQAAVAPNAVAADLVQPGHIFPLQAVDGGVLMRAGHTEAGCDLAAMAGCSPASVIC
EVMNEDGTMARLPDLQIFAAEHDIKIGTIAALIEHRSRVESLVEKVGCREIQTNWGAFTA
HAFLDKPSGGVHLALVRGKWAPEDTVSVRVHEPLSVLDALEINRSLHSWSLDASLSHIAN
EGKGVAVLLNCGETAGELLAQFDGTARPAQAPERGRMDLRSYGIGAQILRECGVHKMNLL
GTPRRMPSMAAGYGLEIAGYLTKD