Protein Info for GFF5698 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: Isochorismatase (EC 3.3.2.1)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00857: Isochorismatase
  • amino acids 3 to 197 (195 residues), 148.7 bits, E=1e-47

Best Hits

KEGG orthology group: None (inferred from 64% identity to psa:PST_1997)

MetaCyc: 47% identical to biuret amidohydrolase subunit (Rhizobium leguminosarum bv. viciae 3841)
Biuret amidohydrolase. [EC: 3.5.1.84]

Predicted SEED Role

"Isochorismatase (EC 3.3.2.1)" in subsystem Chorismate: Intermediate for synthesis of PAPA antibiotics, PABA, anthranilate, 3-hydroxyanthranilate and more. (EC 3.3.2.1)

MetaCyc Pathways

• superpathway of chorismate metabolism (43/59 steps found)
• cyanuric acid degradation II (4/5 steps found)
• 2,3-dihydroxybenzoate biosynthesis (1/3 steps found)
• superpathway of atrazine degradation (4/8 steps found)
• enterobactin biosynthesis (4/11 steps found)
• vibriobactin biosynthesis (1/9 steps found)
• bacillibactin biosynthesis (3/12 steps found)

KEGG Metabolic Maps

• Atrazine degradation
• Biosynthesis of phenylpropanoids
• Biosynthesis of siderophore group nonribosomal peptides

Isozymes

Compare fitness of predicted isozymes for: 3.3.2.1

Use Curated BLAST to search for 3.3.2.1 or 3.5.1.84

Protein Sequence (207 amino acids)

>GFF5698 Isochorismatase (EC 3.3.2.1) (Hydrogenophaga sp. GW460-11-11-14-LB1)
MTTALIVIDMQRDFCSPGGYADTAGMDVQRLREPIARIQQLLTAARAKGLVVVHTREGHL
PDLSDCPPAKLARSTAAGAAIGSQGPLGRLLVRGEQGHDFVDALKPAAGEFVIDKPGYGA
FHQTRLAQLLDAQEVDRLILCGVTTEVCVHSTLREAVDRGFTCTTVGDATAASHPALQAP
ALAMIDVEGGIFGRVRSTAEVVAELGA