Protein Info for GFF5677 in Variovorax sp. SCN45

Annotation: Cytochrome O ubiquinol oxidase subunit I (EC 1.10.3.-)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 667 transmembrane" amino acids 26 to 48 (23 residues), see Phobius details amino acids 68 to 89 (22 residues), see Phobius details amino acids 113 to 137 (25 residues), see Phobius details amino acids 153 to 173 (21 residues), see Phobius details amino acids 199 to 223 (25 residues), see Phobius details amino acids 239 to 264 (26 residues), see Phobius details amino acids 285 to 308 (24 residues), see Phobius details amino acids 320 to 342 (23 residues), see Phobius details amino acids 356 to 378 (23 residues), see Phobius details amino acids 391 to 413 (23 residues), see Phobius details amino acids 425 to 449 (25 residues), see Phobius details amino acids 465 to 485 (21 residues), see Phobius details amino acids 503 to 528 (26 residues), see Phobius details amino acids 599 to 617 (19 residues), see Phobius details amino acids 622 to 640 (19 residues), see Phobius details TIGR02843: cytochrome o ubiquinol oxidase, subunit I" amino acids 12 to 656 (645 residues), 1134.2 bits, see alignment E=0 PF00115: COX1" amino acids 66 to 514 (449 residues), 491.4 bits, see alignment E=1.2e-151

Best Hits

Swiss-Prot: 67% identical to CYOB_ECO57: Cytochrome bo(3) ubiquinol oxidase subunit 1 (cyoB) from Escherichia coli O157:H7

KEGG orthology group: K02298, cytochrome o ubiquinol oxidase subunit I [EC: 1.10.3.-] (inferred from 88% identity to mpt:Mpe_A1743)

MetaCyc: 67% identical to cytochrome bo3 subunit 1 (Escherichia coli K-12 substr. MG1655)
RXN-21817 [EC: 7.1.1.3]

Predicted SEED Role

"Cytochrome O ubiquinol oxidase subunit I (EC 1.10.3.-)" in subsystem Terminal cytochrome O ubiquinol oxidase or Terminal cytochrome oxidases (EC 1.10.3.-)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 1.10.3.-

Use Curated BLAST to search for 1.10.3.- or 7.1.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (667 amino acids)

>GFF5677 Cytochrome O ubiquinol oxidase subunit I (EC 1.10.3.-) (Variovorax sp. SCN45)
MLQNLDLTKLVFGRLTWEAIPYHEPILLATFAGVVLGGIAVLGALTYFKLWGMLWRDWIT
SIDHKKIGIMYIILGLVMLLRGFADALMMRAQQAMAFGDNAGFLPPHHYDQVFTAHGVIM
IFFVAMPLVTGLMNFVVPLQIGARDVAFPFLNNFSFWMTTFGAGLVMASLFVGEFAKTGW
LAYPPLSGILYSPDVGVDYYIWSLQIAGVGTLLSGVNLLATIVKMRAPGMTMMKMPVFTW
TALCTNVLIVAAFPVLTAVLALLSLDRYAGTNFFTNDMGGNAMMYVNLIWIWGHPEVYIL
ILPAFGIFSEVVSTFSGKRLFGYASMVYATVVITILSYLVWLHHFFTMGSGASVNSFFGI
TTMIISIPTGAKIFNWLFTMYRGRIRFELPMMWTIGFMITFVIGGMTGVLLAVPPADFVL
HNSLFLIAHFHNVIIGGVLFGMLAGITYWFPKAFGYKLDPFWGKCSFWFWIVGFWFAFMP
LYILGLMGVTRRMSHFQDMSLQIWFQVAAFGAVLIALGIASFIIQLVVSFIRRESLRDTT
GDPWNGRTLEWSTSSPPPAYNFAFTPRVHDNDAWSDMKARGYERPLEGFSPIHMPKNTAA
GFIIAALAAVCGFALIWQMWLVAGIGFVAMLAATIIHTFNYKRDYHIPTEEVVRTEDART
RLLAANV