Protein Info for GFF564 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: UDP-N-acetylmuramate--alanine ligase (EC 6.3.2.8)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01225: Mur_ligase
  • amino acids 7 to 105 (99 residues), 112 bits, E=2.2e-36
• TIGR01082: UDP-N-acetylmuramate--L-alanine ligase
  • amino acids 7 to 458 (452 residues), 550.7 bits, E=1.5e-169
• PF08245: Mur_ligase_M
  • amino acids 110 to 293 (184 residues), 88.7 bits, E=7.8e-29
• PF02875: Mur_ligase_C
  • amino acids 313 to 402 (90 residues), 53.2 bits, E=4.7e-18

Best Hits

Swiss-Prot: 78% identical to MURC_RHOFT: UDP-N-acetylmuramate--L-alanine ligase (murC) from Rhodoferax ferrireducens (strain ATCC BAA-621 / DSM 15236 / T118)

KEGG orthology group: K01924, UDP-N-acetylmuramate--alanine ligase [EC: 6.3.2.8] (inferred from 80% identity to vpe:Varpa_0980)

MetaCyc: 54% identical to UDP-N-acetylmuramate--L-alanine ligase (Escherichia coli K-12 substr. MG1655)
UDP-N-acetylmuramate--L-alanine ligase. [EC: 6.3.2.8]

Predicted SEED Role

"UDP-N-acetylmuramate--alanine ligase (EC 6.3.2.8)" in subsystem Peptidoglycan Biosynthesis (EC 6.3.2.8)

MetaCyc Pathways

• peptidoglycan biosynthesis I (meso-diaminopimelate containing) (11/12 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis I (meso-diaminopimelate containing) (8/8 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis II (lysine-containing) (7/8 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis III (meso-diaminopimelate containing) (6/8 steps found)
• peptidoglycan biosynthesis III (mycobacteria) (11/15 steps found)
• peptidoglycan biosynthesis II (staphylococci) (12/17 steps found)
• peptidoglycan biosynthesis IV (Enterococcus faecium) (12/17 steps found)
• peptidoglycan biosynthesis V (β-lactam resistance) (11/17 steps found)

KEGG Metabolic Maps

• D-Glutamine and D-glutamate metabolism
• Peptidoglycan biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.8

Protein Sequence (469 amino acids)

>GFF564 UDP-N-acetylmuramate--alanine ligase (EC 6.3.2.8) (Hydrogenophaga sp. GW460-11-11-14-LB1)
MKHAIRHIHFVGIGGAGMSGIAEVLLNQGYRISGSDLGESTVTRRLVALGAQVFVGHDAA
NIEGADAIVTSTAVKPDNPEVVAAHAKLVPVVPRAVMLAELMRMKKGVAIAGTHGKTTTT
SLVASVLAAAQLDPTFVIGGRLNSAGANAQLGSGEYIVVEADESDASFLNLLPVLSVVTN
IDADHMDTYGHDFGNLKAAFVDFLHKMPFYGAAVVCVDDAAIREILPQIARPITSYGFSE
DAQVRAINVRAVDTQMHFTVQRRNGVELPDLDVVLNLPGNHNVLNALAAIGIAVELNVPD
EAVQRALAEFKGVGRRFQRYGEAALPSGGTCTVVDDYGHHPVEMAATLSAARGAYPGRRL
VLAFQPHRYTRTRDCFEDFVKVIGQADAVLLAEVYAAGEAPIVAADGRSLARALRVAGKL
EPVFVDDIAAMPQAVLDNARDGDVVLCMGAGTVGAVAGRVIELAQGAHA