Protein Info for GFF5632 in Variovorax sp. SCN45

Annotation: tRNA (adenine(37)-N6)-methyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 165 TIGR00104: tRNA-Thr(GGU) m(6)t(6)A37 methyltransferase TsaA" amino acids 10 to 141 (132 residues), 117 bits, see alignment E=3.8e-38 PF01980: TrmO_N" amino acids 26 to 138 (113 residues), 128.5 bits, see alignment E=7.5e-42

Best Hits

Swiss-Prot: 48% identical to Y663_PYRAB: Probable S-adenosyl-L-methionine-binding protein PYRAB06630 (PYRAB06630) from Pyrococcus abyssi (strain GE5 / Orsay)

KEGG orthology group: None (inferred from 74% identity to del:DelCs14_2589)

Predicted SEED Role

"COG1720: Uncharacterized conserved protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (165 amino acids)

>GFF5632 tRNA (adenine(37)-N6)-methyltransferase (Variovorax sp. SCN45)
MKPEALEPVVCRPVGVVRSRFTEAAGMPIQTAGAPGEPGRLEVFAEFAPGLRDIEGFDYL
ILVTHLHQCAHERLEVVPFLDNASHGVFATRAPARPNRLGLSIVRLLSVDGTTLHFSGND
MMDGTPVLDIKPYVPRFDVRETERVGWFGARLDQLPSTRSDDRMG