Protein Info for GFF5624 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: L-lactate dehydrogenase (EC 1.1.2.3)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 419 PF01070: FMN_dh" amino acids 42 to 401 (360 residues), 421.4 bits, see alignment E=2.8e-130

Best Hits

Swiss-Prot: 55% identical to LLDD_MYCTU: Putative L-lactate dehydrogenase (lldD) from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

KEGG orthology group: K00101, L-lactate dehydrogenase (cytochrome) [EC: 1.1.2.3] (inferred from 60% identity to cfi:Celf_3326)

Predicted SEED Role

"L-lactate dehydrogenase (EC 1.1.2.3)" in subsystem L-rhamnose utilization or Lactate utilization or Respiratory dehydrogenases 1 (EC 1.1.2.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.2.3

Use Curated BLAST to search for 1.1.2.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (419 amino acids)

>GFF5624 L-lactate dehydrogenase (EC 1.1.2.3) (Hydrogenophaga sp. GW460-11-11-14-LB1)
MQRQFPRWRELRPLLSFQRFEWNGTARRLAKAHTIDDLRAIARRRTPAGPFHYVDGAADE
EITLGRVRQYYRDLEFQPGVLRDVSVTDPGTELFGRALRLPFGFAPTGFTRLLHAEGERA
VVRAAQAKGIPYALSTMGTTGITELAQAAPHARRWFQLYLWKDRERSLRFIEQARAAGYD
ALVVTVDVPTAGNRLRDLRHGMTMPPRLSLKTLLDASHRPNWWFDFLTTPPPRFAFASAG
EHEGAAELVAKLYDTSATVEDLRWMREAWNGPLVVKGIQTLEDARRVADFGADGIVLSNH
GGRQLDRAPIPLRLLPEVVDAVGRQTAVMIDSGIQHGADIVAALALGARFAWVGRAYLYG
LMAGGEAGVLRAADILESQIVRTLKLLGVQRIDQLHRDHVRLLEAQAFTTRRTHVEVAR