Protein Info for GFF560 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: Phospho-N-acetylmuramoyl-pentapeptide- transferase (EC 2.7.8.13)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 392 signal peptide" amino acids 1 to 18 (18 residues), see Phobius details transmembrane" amino acids 27 to 48 (22 residues), see Phobius details amino acids 73 to 94 (22 residues), see Phobius details amino acids 100 to 117 (18 residues), see Phobius details amino acids 137 to 157 (21 residues), see Phobius details amino acids 193 to 213 (21 residues), see Phobius details amino acids 225 to 245 (21 residues), see Phobius details amino acids 265 to 282 (18 residues), see Phobius details amino acids 289 to 310 (22 residues), see Phobius details amino acids 316 to 339 (24 residues), see Phobius details amino acids 370 to 389 (20 residues), see Phobius details TIGR00445: phospho-N-acetylmuramoyl-pentapeptide-transferase" amino acids 41 to 392 (352 residues), 402.3 bits, see alignment E=9.1e-125 PF00953: Glycos_transf_4" amino acids 102 to 310 (209 residues), 105.9 bits, see alignment E=1.1e-34

Best Hits

Swiss-Prot: 86% identical to MRAY_POLNA: Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY) from Polaromonas naphthalenivorans (strain CJ2)

KEGG orthology group: K01000, phospho-N-acetylmuramoyl-pentapeptide-transferase [EC: 2.7.8.13] (inferred from 86% identity to pna:Pnap_3420)

Predicted SEED Role

"Phospho-N-acetylmuramoyl-pentapeptide-transferase (EC 2.7.8.13)" in subsystem Peptidoglycan Biosynthesis (EC 2.7.8.13)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.8.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (392 amino acids)

>GFF560 Phospho-N-acetylmuramoyl-pentapeptide- transferase (EC 2.7.8.13) (Hydrogenophaga sp. GW460-11-11-14-LB1)
MLPSLALWLQGLSPEFGSLRVFQYLTFRAVMAAMTALLVGLVAGPYFIRRLAALKIGQPI
REYAMQTHLSKGGTPTMGGVLILFSIALSTLLWFDLTNRFVWIVLMVTLGFGAIGWVDDW
RKVVHKDPEGMRSREKYFWQSVIGLVAAFYLVFAVSGTSNERVFELFVAWVRSGFTLDMP
PQAGLLVPFFKEISYPLGVFGFVIMTYLVIVGSSNAVNLTDGLDGLAIMPVVMVGSALGV
FAYVTGNSVFARYLLLPHIPGAGELLIFCAAMAGAGLAFLWFNTHPAQVFMGDVGALALG
GALGTVAVIVRQEIVLAIMGGIFVVEALSVMLQVTWFKYTKRRYGTGRRLFQMAPLHHHF
EKKGWKETQVVVRFWIITMLLCLVGLSTLKLR