Protein Info for GFF5587 in Variovorax sp. SCN45

Annotation: Acetoin dehydrogenase E1 component alpha-subunit (EC 2.3.1.190)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00676: E1_dh
  • amino acids 42 to 321 (280 residues), 207.7 bits, E=3.1e-65
• PF02775: TPP_enzyme_C
  • amino acids 124 to 208 (85 residues), 25.9 bits, E=1.1e-09
• PF13292: DXP_synthase_N
  • amino acids 129 to 199 (71 residues), 26 bits, E=7.9e-10

Best Hits

Swiss-Prot: 36% identical to ODPA_RHIME: Pyruvate dehydrogenase E1 component subunit alpha (pdhA) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K00161, pyruvate dehydrogenase E1 component subunit alpha [EC: 1.2.4.1] (inferred from 92% identity to vap:Vapar_5030)

Predicted SEED Role

"Pyruvate dehydrogenase E1 component alpha subunit (EC 1.2.4.1)" in subsystem Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate (EC 1.2.4.1)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (24/26 steps found)
• superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (20/22 steps found)
• TCA cycle II (plants and fungi) (9/9 steps found)
• superpathway of glyoxylate bypass and TCA (11/12 steps found)
• TCA cycle I (prokaryotic) (9/10 steps found)
• TCA cycle III (animals) (9/10 steps found)
• TCA cycle VIII (Chlamydia) (6/6 steps found)
• pyruvate fermentation to acetate and (S)-lactate I (4/4 steps found)
• pyruvate decarboxylation to acetyl CoA I (3/3 steps found)
• pyruvate fermentation to acetate II (3/3 steps found)
• superpathway of anaerobic energy metabolism (invertebrates) (13/17 steps found)
• pyruvate fermentation to acetate V (2/3 steps found)
• 1-butanol autotrophic biosynthesis (engineered) (19/27 steps found)
• photosynthetic 3-hydroxybutanoate biosynthesis (engineered) (18/26 steps found)
• anaerobic energy metabolism (invertebrates, mitochondrial) (6/10 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Butanoate metabolism
• Citrate cycle (TCA cycle)
• Glycolysis / Gluconeogenesis
• Pyruvate metabolism
• Valine, leucine and isoleucine biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 1.2.4.1

Use Curated BLAST to search for 1.2.4.1

Protein Sequence (338 amino acids)

>GFF5587 Acetoin dehydrogenase E1 component alpha-subunit (EC 2.3.1.190) (Variovorax sp. SCN45)
MDPSTSDAQALYRVMVRIRAFENAAEAASQGGVSAYGQQAAGAAKVRGPLHLSTGQEAVP
AGVCAHLRASDYLTSTHRGHGHTLAKGADLARMMCELFGKATGFNGGKGGSMHIADFSVG
MLGANGVVAAGLPIAVGAAHAQKLLQTRDDITVCFFGDGAINRGPFLEALNWARVYELPV
LFVCEDNRWSATTASGPMTAGEGASARAESLGIAATQVDGNDVFAVHETAARLVTEVRAG
GGPRLLHALTYRVKGHVSVDLAAYRDPAELAAALETDPIARARGHLLSMGVAAAALDAIE
NAARDEVDTALAIADAAPWPDAGAAFTDVQTTGAGQWH