Protein Info for GFF558 in Hydrogenophaga sp. GW460-11-11-14-LB1
Annotation: UDP-N-acetylmuramoylalanyl-D-glutamate--2,6- diaminopimelate ligase (EC 6.3.2.13)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K01928, UDP-N-acetylmuramoylalanyl-D-glutamate--2,6-diaminopimelate ligase [EC: 6.3.2.13] (inferred from 54% identity to lch:Lcho_0517)Predicted SEED Role
"UDP-N-acetylmuramoylalanyl-D-glutamate--2,6-diaminopimelate ligase (EC 6.3.2.13)" in subsystem Methicillin resistance in Staphylococci or Peptidoglycan Biosynthesis (EC 6.3.2.13)
MetaCyc Pathways
- peptidoglycan biosynthesis I (meso-diaminopimelate containing) (11/12 steps found)
- UDP-N-acetylmuramoyl-pentapeptide biosynthesis I (meso-diaminopimelate containing) (8/8 steps found)
- UDP-N-acetylmuramoyl-pentapeptide biosynthesis III (meso-diaminopimelate containing) (6/8 steps found)
- peptidoglycan biosynthesis III (mycobacteria) (11/15 steps found)
KEGG Metabolic Maps
Isozymes
No predicted isozymesUse Curated BLAST to search for 6.3.2.13
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (513 amino acids)
>GFF558 UDP-N-acetylmuramoylalanyl-D-glutamate--2,6- diaminopimelate ligase (EC 6.3.2.13) (Hydrogenophaga sp. GW460-11-11-14-LB1) MRTIHQPQAVADWLRERGVRALHCDSRRVGAGDAFVAWPGAATDGRRFVAGALQAGAVAA LVEQDGAQAYGFDDERVVAVPGLKASAGAIAGAFHGTPSAALDMVAITGTNGKTSTAWWT AQLLSFCAMPCAVVGTLGMGRPPRPGQADSPLVATGLTTPDPVMLQAELRRFVDSGLKAC AVEASSIGIVEGRLNATQLRVAVFTNFTQDHLDFHGSMEAYWTAKAELFDWPGLASAVVN IDDARGADLAATLAQRGLDLWTVAIEAGHHGRARLFAQDLSFTHSGMALQVGERDARGRT LAQHALSLPLVGRYNVSNLLCVLASARALGVALADAVQACAALTPVPGRMEQAAAGVSDQ PLVLVDYAHTPDALEKALQALQPLARQRGGALWVVVGCGGDRDAGKRPLMAAAAEREAQR PVLTSDNPRSEDPLAILRQMVAGLASPDAALVEPDRAQAIALAVARADARDVVLIAGKGH EDYQDVKGVKKPFSDLLHARAALAQRRGQGAPA