Protein Info for PS417_28470 in Pseudomonas simiae WCS417

Annotation: ATP F0F1 synthase subunit gamma

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 286 TIGR01146: ATP synthase F1, gamma subunit" amino acids 1 to 286 (286 residues), 354 bits, see alignment E=4.2e-110 PF00231: ATP-synt" amino acids 4 to 286 (283 residues), 352.5 bits, see alignment E=1.1e-109

Best Hits

Swiss-Prot: 99% identical to ATPG_PSEPF: ATP synthase gamma chain (atpG) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K02115, F-type H+-transporting ATPase subunit gamma [EC: 3.6.3.14] (inferred from 99% identity to pfl:PFL_6217)

MetaCyc: 69% identical to ATP synthase F1 complex subunit gamma (Escherichia coli K-12 substr. MG1655)
ATPSYN-RXN [EC: 7.1.2.2]; RXN0-7041 [EC: 7.1.2.2]

Predicted SEED Role

"ATP synthase gamma chain (EC 3.6.3.14)" in subsystem F0F1-type ATP synthase (EC 3.6.3.14)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.14

Use Curated BLAST to search for 3.6.3.14 or 7.1.2.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7UEQ7 at UniProt or InterPro

Protein Sequence (286 amino acids)

>PS417_28470 ATP F0F1 synthase subunit gamma (Pseudomonas simiae WCS417)
MAGAKEIRSKIASIKSTQKITSAMEKVAVSKMRKAQMRMAASRPYAERIRQVIGHLANAN
PEYRHPFMIDREVKRVGYVVVSSDRGLCGGLNTNLFKALVKDMAVNREKGVEIDLCVVGS
KGAAFFRNFGGNVVAAISHLGEEPSINDLIGSVKVMLDAYLEGRIDRLSVVSNKFINTMT
QQPTVEQLIPLVATPDQELKHHWDYLYEPDAKELLDGLMVRYVESQVYQAVVENNAAEQA
ARMIAMKNATDNAGDLISDLQLIYNKARQAAITQEISEIVGGAAAV