Protein Info for PS417_28445 in Pseudomonas simiae WCS417

Annotation: glucosamine--fructose-6-phosphate aminotransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01135: glutamine-fructose-6-phosphate transaminase (isomerizing)
  • amino acids 2 to 610 (609 residues), 859.4 bits, E=7.4e-263
• PF13522: GATase_6
  • amino acids 67 to 181 (115 residues), 67 bits, E=2.7e-22
• PF13537: GATase_7
  • amino acids 84 to 193 (110 residues), 50.7 bits, E=2.7e-17
• PF01380: SIS
  • amino acids 290 to 417 (128 residues), 111.2 bits, E=4.8e-36
  • amino acids 463 to 592 (130 residues), 93.9 bits, E=1.1e-30

Best Hits

Swiss-Prot: 89% identical to GLMS_PSESM: Glutamine--fructose-6-phosphate aminotransferase [isomerizing] (glmS) from Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000)

KEGG orthology group: K00820, glucosamine--fructose-6-phosphate aminotransferase (isomerizing) [EC: 2.6.1.16] (inferred from 99% identity to pfs:PFLU6114)

MetaCyc: 63% identical to L-glutamine--D-fructose-6-phosphate aminotransferase (Escherichia coli K-12 substr. MG1655)
Glutamine--fructose-6-phosphate transaminase (isomerizing). [EC: 2.6.1.16]

Predicted SEED Role

"Glucosamine--fructose-6-phosphate aminotransferase [isomerizing] (EC 2.6.1.16)" in subsystem Sialic Acid Metabolism or UDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis (EC 2.6.1.16)

MetaCyc Pathways

• O-antigen building blocks biosynthesis (E. coli) (10/11 steps found)
• UDP-N-acetyl-D-glucosamine biosynthesis I (5/5 steps found)
• UDP-N-acetyl-D-glucosamine biosynthesis II (4/6 steps found)
• chitin biosynthesis (5/9 steps found)
• UDP-N-acetyl-D-galactosamine biosynthesis III (2/6 steps found)
• CMP-legionaminate biosynthesis I (2/10 steps found)
• superpathway of UDP-N-acetylglucosamine-derived O-antigen building blocks biosynthesis (9/24 steps found)
• superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (12/33 steps found)

KEGG Metabolic Maps

• Aminosugars metabolism
• Glutamate metabolism
• Nucleotide sugars metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.6.1.16

Use Curated BLAST to search for 2.6.1.16

Sequence Analysis Tools

See A0A1N7UK01 at UniProt or InterPro

Protein Sequence (610 amino acids)

>PS417_28445 glucosamine--fructose-6-phosphate aminotransferase (Pseudomonas simiae WCS417)
MCGIVGAVAERNITPILIEGLKRLEYRGYDSAGVAVFTNAGKLERMRRPGKVSELEQALN
GEPLVGRLGIAHTRWATHGAPCERNAHPHFSGDLAVVHNGIIENHEVLREQLKGQGYVFT
SDTDTEVIAHLLNHKLKDLGDLTVALKATVKELHGAYGLAVVSASQPDRVVAARSGSPLV
IGLGLGENFLASDQLALRQVTDRFMYLEEGDIADIRRESVQIWDVNGQSVEREAVQYRDG
AEAADKGEFRHFMLKEIHEQPSVVQRTLEGRLSQNQVLVNAFGPQAAELFAKVRNVQIVA
CGTSYHAGMVARYWLEELAGIPCQVEVASEFRYRKVVVQPDTLFVTISQSGETADTLAAL
RNAKELGFLASLAICNVSISSLVRESDLTLLTQAGREIGVASTKAFTTQLVGLLLLTLSL
GQVRGTLAEGVEATLVEELRRLPARLGEALAMDSTVEKVAELFADKNHTLFLGRGAQYPV
AMEGSLKLKEISYIHAEAYPAGELKHGPLALVDDDMPVVTVAPNNELLEKLKSNLQEVRA
RGGQLVVFADEKAGMTNGEGTHVINMPHINDTLSPILYTIPLQLLSYYVAVLKGTDVDQP
RNLAKSVTVE