Protein Info for GFF555 in Sphingobium sp. HT1-2

Annotation: 2-keto-4-pentenoate hydratase (EC 4.2.1.80)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01557: FAA_hydrolase
  • amino acids 84 to 251 (168 residues), 75.4 bits, E=2.6e-25

Best Hits

Swiss-Prot: 59% identical to MHPD2_PSEPU: 2-keto-4-pentenoate hydratase 2 (mhpD2) from Pseudomonas putida

KEGG orthology group: K02554, 2-keto-4-pentenoate hydratase [EC: 4.2.1.80] (inferred from 59% identity to ppw:PputW619_1982)

MetaCyc: 55% identical to 2-hydroxypentadienoate hydratase (Escherichia coli K-12 substr. MG1655)
2-oxopent-4-enoate hydratase. [EC: 4.2.1.80]

Predicted SEED Role

"2-keto-4-pentenoate hydratase (EC 4.2.1.80)" (EC 4.2.1.80)

MetaCyc Pathways

• 2-hydroxypenta-2,4-dienoate degradation (3/3 steps found)
• catechol degradation I (meta-cleavage pathway) (3/5 steps found)
• mandelate degradation to acetyl-CoA (11/18 steps found)
• catechol degradation II (meta-cleavage pathway) (3/7 steps found)
• toluene degradation I (aerobic) (via o-cresol) (3/7 steps found)
• toluene degradation V (aerobic) (via toluene-cis-diol) (3/7 steps found)
• meta cleavage pathway of aromatic compounds (5/10 steps found)
• 3-phenylpropanoate and 3-(3-hydroxyphenyl)propanoate degradation (3/8 steps found)
• p-cumate degradation (3/8 steps found)
• toluene degradation II (aerobic) (via 4-methylcatechol) (1/6 steps found)
• naphthalene degradation to acetyl-CoA (5/12 steps found)
• toluene degradation IV (aerobic) (via catechol) (5/13 steps found)
• L-tryptophan degradation IX (4/12 steps found)
• L-tryptophan degradation XII (Geobacillus) (4/12 steps found)
• p-cymene degradation (3/11 steps found)
• 4-chloronitrobenzene degradation (1/9 steps found)
• superpathway of aromatic compound degradation via 3-oxoadipate (17/35 steps found)
• superpathway of aerobic toluene degradation (10/30 steps found)
• superpathway of aromatic compound degradation via 2-hydroxypentadienoate (13/42 steps found)

KEGG Metabolic Maps

• 1,4-Dichlorobenzene degradation
• Benzoate degradation via hydroxylation
• Biphenyl degradation
• Carbazole degradation
• Ethylbenzene degradation
• Fluorene degradation
• Phenylalanine metabolism
• Styrene degradation
• Toluene and xylene degradation

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.80

Use Curated BLAST to search for 4.2.1.80

Protein Sequence (254 amino acids)

>GFF555 2-keto-4-pentenoate hydratase (EC 4.2.1.80) (Sphingobium sp. HT1-2)
MMTAEIGALLRDAYDGAPIPPVADRIASVEDAYQVQLWQIAERLAAGRRIVGRKIGLTSP
AVQRQLGVDEPDFGHLTDEMVHGDNARLVHATLRQPRIEAEVALILGADLATPHATVADV
IAATAYVVPALEVVASRITDWKIGILDTVADNASAGAVVLGGPARRLDGIDLTHCAMTMT
INGEMVSQGQGSDCLGSPLNAATWLARRCHSLGRPLLAGEIILTGALGPMRAVAPGDDVH
AEIDGLGTVSASFC