Protein Info for GFF5548 in Variovorax sp. SCN45

Annotation: Urea carboxylase (EC 6.3.4.6) without Allophanate hydrolase 2 domains

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00289: Biotin_carb_N
  • amino acids 1 to 109 (109 residues), 143.4 bits, E=1.1e-45
• PF02786: CPSase_L_D2
  • amino acids 114 to 321 (208 residues), 254.5 bits, E=2.2e-79
• PF02222: ATP-grasp
  • amino acids 137 to 291 (155 residues), 29.4 bits, E=1.9e-10
• PF07478: Dala_Dala_lig_C
  • amino acids 143 to 290 (148 residues), 25.8 bits, E=2.2e-09
• PF02785: Biotin_carb_C
  • amino acids 334 to 443 (110 residues), 119 bits, E=3.1e-38
• PF00364: Biotin_lipoyl
  • amino acids 515 to 578 (64 residues), 41.7 bits, E=2.4e-14

Best Hits

Swiss-Prot: 62% identical to BCCA_MYCLE: Acetyl-/propionyl-coenzyme A carboxylase alpha chain (bccA) from Mycobacterium leprae (strain TN)

KEGG orthology group: K11263, acetyl-/propionyl-CoA carboxylase, biotin carboxylase, biotin carboxyl carrier protein [EC: 6.3.4.14] (inferred from 86% identity to vpe:Varpa_0701)

MetaCyc: 65% identical to acetyl/propionyl-CoA carboxylase alpha chain (Mycobacterium tuberculosis H37Rv)
RXN0-5055 [EC: 2.1.3.15]

Predicted SEED Role

"Urea carboxylase (EC 6.3.4.6) without Allophanate hydrolase 2 domains" (EC 6.3.4.6)

MetaCyc Pathways

• superpathway of fatty acids biosynthesis (E. coli) (48/53 steps found)
• superpathway of fatty acid biosynthesis II (plant) (38/43 steps found)
• superpathway of fatty acid biosynthesis I (E. coli) (14/16 steps found)
• biotin-carboxyl carrier protein assembly (4/4 steps found)
• fatty acid biosynthesis initiation (type II) (3/3 steps found)
• urea degradation I (3/3 steps found)
• superpathway of fatty acid biosynthesis initiation (4/5 steps found)
• 3-hydroxypropanoate cycle (9/13 steps found)
• superpathway of allantoin degradation in yeast (3/6 steps found)
• 3-hydroxypropanoate/4-hydroxybutanate cycle (11/18 steps found)
• superpathway of the 3-hydroxypropanoate cycle (11/18 steps found)
• glyoxylate assimilation (7/13 steps found)
• candicidin biosynthesis (1/6 steps found)
• superpathway of candicidin biosynthesis (4/11 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Fatty acid biosynthesis
• Urea cycle and metabolism of amino groups

Isozymes

Compare fitness of predicted isozymes for: 6.3.4.14

Use Curated BLAST to search for 2.1.3.15 or 6.3.4.14 or 6.3.4.6

Protein Sequence (579 amino acids)

>GFF5548 Urea carboxylase (EC 6.3.4.6) without Allophanate hydrolase 2 domains (Variovorax sp. SCN45)
MKKVLIANRGEIAVRIARACADYGVKSVAVYADADIDALHVRMADEAYGLEGDRPADTYL
HIAKLLAVAARSGADAVHPGYGFLSESAAFAQAVIDAGLTWVGPSPAAIARLGDKVQARK
LAMKVGAPLVAGTADPVQSAAEVLAFAEKHGLPVAIKAAFGGGGRGIKVAWRMDEVEGLY
ESAVREAVTAFGRGECYVEQFLDRPRHVEAQVLGDTHGNVVVLGTRDCSLQRRNQKLVEE
APAPFLSDAQRERIHQSARDICAEAGYTGAGTVEFMLSASGAISFLEVNTRLQVEHPVTE
ETTGIDLVVEQLRIADGLPLSVTDTPAPRGHAFEFRINAEDVGRGFLPTPGPVKVFDVPS
GPGVRIDTGVAAGSTVPGTFDSLMAKLIVTGATRAQAVARARRALAEFRIEGVASVLPFH
RAAMAHPDFVSGDAFKVHTRWIETDFANGETPAERPEPRPETALVRTAIEVDGRRLSLGL
PPELLRGLSSAHAGSAGGAPSAEAVVAVQDPAAVAAPVSGTLQGWKVADGEEVAQGAVIA
VMEAMKMEMQVLAHRAGRIAFGVAAGGYVTVGATIATIG