Protein Info for GFF5543 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: Cobalt-containing nitrile hydratase subunit alpha (EC 4.2.1.84)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02979: NHase_alpha
  • amino acids 21 to 201 (181 residues), 294.9 bits, E=9.1e-93
• TIGR01323: nitrile hydratase, alpha subunit
  • amino acids 21 to 206 (186 residues), 304.4 bits, E=2.5e-95

Best Hits

Swiss-Prot: 80% identical to NHAA_PSEPU: Nitrile hydratase subunit alpha (nthA) from Pseudomonas putida

KEGG orthology group: K01721, nitrile hydratase [EC: 4.2.1.84] (inferred from 78% identity to bpt:Bpet1416)

MetaCyc: 57% identical to nitrile hydratase alpha subunit (Rhodococcus rhodochrous)
Nitrile hydratase. [EC: 4.2.1.84]

Predicted SEED Role

"Cobalt-containing nitrile hydratase subunit alpha (EC 4.2.1.84)" (EC 4.2.1.84)

MetaCyc Pathways

• acrylonitrile degradation I (2/2 steps found)
• indole-3-acetate biosynthesis IV (bacteria) (2/2 steps found)
• aldoxime degradation (2/3 steps found)
• superpathway of acrylonitrile degradation (2/3 steps found)
• indole-3-acetate biosynthesis II (4/12 steps found)

KEGG Metabolic Maps

• 1,4-Dichlorobenzene degradation
• Benzoate degradation via CoA ligation
• Cyanoamino acid metabolism
• Styrene degradation
• Tryptophan metabolism

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.84

Use Curated BLAST to search for 4.2.1.84

Protein Sequence (211 amino acids)

>GFF5543 Cobalt-containing nitrile hydratase subunit alpha (EC 4.2.1.84) (Hydrogenophaga sp. GW460-11-11-14-LB1)
VSHSHYDDHHHDGYQAPPEGMELRVKALESLLVEKGLIDQAAMEAVVDTYENKVGPRNGA
KVVAKAWVDADYKKRLLEDATKAIAELGFSGAQGEDIVVVENTASVHNVFVCTLCSCYPW
PTLGLPPVWYKAAPYRSRMVIDPRGLLAEFGLDVPADKEIRVWDTTAELRYMVLPERPVG
SQDLTEEQLAELVTRDSMIGIGMPMQPTSTK