Protein Info for GFF5535 in Variovorax sp. SCN45

Annotation: S-adenosylmethionine:tRNA ribosyltransferase-isomerase (EC 2.4.99.17)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 347 transmembrane" amino acids 307 to 324 (18 residues), see Phobius details TIGR00113: S-adenosylmethionine:tRNA ribosyltransferase-isomerase" amino acids 4 to 342 (339 residues), 408.8 bits, see alignment E=8.5e-127 PF02547: Queuosine_synth" amino acids 6 to 342 (337 residues), 417.7 bits, see alignment E=1.6e-129

Best Hits

Swiss-Prot: 55% identical to QUEA_AZOSB: S-adenosylmethionine:tRNA ribosyltransferase-isomerase (queA) from Azoarcus sp. (strain BH72)

KEGG orthology group: K07568, S-adenosylmethionine:tRNA ribosyltransferase-isomerase [EC: 5.-.-.-] (inferred from 93% identity to vpe:Varpa_5789)

Predicted SEED Role

"S-adenosylmethionine:tRNA ribosyltransferase-isomerase (EC 5.-.-.-)" in subsystem Queuosine-Archaeosine Biosynthesis (EC 5.-.-.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 5.-.-.-

Use Curated BLAST to search for 5.-.-.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (347 amino acids)

>GFF5535 S-adenosylmethionine:tRNA ribosyltransferase-isomerase (EC 2.4.99.17) (Variovorax sp. SCN45)
MRDFTLSDFDFALPPELVAQHPATERTSSRLLDGTGPAPVDRIFKNLPSLLRAGDLLVFN
DTRVVKARLFGEKPTGGKLELLVERVLQGQEVVAHMKVSKKPPVGTTLDMVGGFRATLLG
RWPDEDGALFRFAFESDTGENPYMLMERCGHVPLPPYITHTDSAEDESRYQTVFARVPGA
VAAPTAALHFDEGLLAELEARGVQRASVTLHVGAGTFQPVKTENISEHTMHAERYEVPEA
TQRAIAECKARGGRIVAVGTTTVRTLESWAKSGEASGDTRIFITPGFAFAHVDLLVTNFH
LPKSTLMMLVSALAGYERVMALYAHAIAERYRFFSYGDAMLLARQQD