Protein Info for GFF5514 in Variovorax sp. SCN45

Annotation: Threonylcarbamoyl-AMP synthase (EC 2.7.7.87) / SUA5 domain with internal deletion

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 333 TIGR00057: tRNA threonylcarbamoyl adenosine modification protein, Sua5/YciO/YrdC/YwlC family" amino acids 7 to 208 (202 residues), 159.1 bits, see alignment E=4.1e-51 PF01300: Sua5_yciO_yrdC" amino acids 17 to 199 (183 residues), 169.8 bits, see alignment E=4.4e-54 PF03481: Sua5_C" amino acids 201 to 332 (132 residues), 88.9 bits, see alignment E=4.4e-29

Best Hits

KEGG orthology group: K07566, putative translation factor (inferred from 91% identity to vpe:Varpa_5809)

Predicted SEED Role

"TsaC protein (YrdC domain) required for threonylcarbamoyladenosine t(6)A37 modification in tRNA"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (333 amino acids)

>GFF5514 Threonylcarbamoyl-AMP synthase (EC 2.7.7.87) / SUA5 domain with internal deletion (Variovorax sp. SCN45)
MILDGRSPDAIAEAVRVLRAGGLVAFPTETVYGLGADASCDTAVAGIFKAKGRPADHPLI
VHVAAGTKGAESLSRFAQPLPPFAQKLVQAFWPGPLTLIVTRQPGVGAAAAGGQDTIGLR
CPSHPVAQALLLACAEQGVPGLAGPSANRFGRVSPTTAQHVYDEFGDALPVVDGGACEVG
IESTIVDCSRGAPVLLRPGLITRAQIEAACGEKLADREVLETPDPRASGTLEAHYAPDAK
VRLMDAKAIQTGLDVLGANAAHIAVWSRSVVRSRSQRVLLRRMPDDAATSAQQLFAVLRA
FDTEGVKLIWVETPPDTPEWEGVKDRLQRAAAA