Protein Info for PS417_28210 in Pseudomonas simiae WCS417

Annotation: pyruvate carboxylase subunit B

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01108: oxaloacetate decarboxylase alpha subunit
  • amino acids 7 to 597 (591 residues), 837.1 bits, E=3.6e-256
• PF00682: HMGL-like
  • amino acids 7 to 267 (261 residues), 162.7 bits, E=2.7e-51
• PF02436: PYC_OADA
  • amino acids 292 to 482 (191 residues), 194.9 bits, E=2.7e-61
• PF00364: Biotin_lipoyl
  • amino acids 535 to 600 (66 residues), 58.5 bits, E=9.4e-20
• PF13533: Biotin_lipoyl_2
  • amino acids 571 to 601 (31 residues), 30.7 bits, see alignment (E = 4.3e-11)

Best Hits

KEGG orthology group: K01960, pyruvate carboxylase subunit B [EC: 6.4.1.1] (inferred from 99% identity to pfs:PFLU6070)

Predicted SEED Role

No annotation

MetaCyc Pathways

• gluconeogenesis III (8/12 steps found)
• reductive TCA cycle I (6/11 steps found)
• incomplete reductive TCA cycle (3/7 steps found)
• gluconeogenesis II (Methanobacterium thermoautotrophicum) (9/18 steps found)
• (S)-lactate fermentation to propanoate, acetate and hydrogen (5/13 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (20/56 steps found)

KEGG Metabolic Maps

• Alanine and aspartate metabolism
• Citrate cycle (TCA cycle)
• Pyruvate metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.4.1.1

Use Curated BLAST to search for 6.4.1.1

Sequence Analysis Tools

See A0A1N7U628 at UniProt or InterPro

Protein Sequence (602 amino acids)

>PS417_28210 pyruvate carboxylase subunit B (Pseudomonas simiae WCS417)
MSKKIFVTDTILRDAHQSLLATRMRTDDMLPICDKLDKVGYWSLEVWGGATFDACVRFLK
EDPWERLRKLRAALPNTRLQMLLRGQNLLGYRHYSDDVVKAFVAKAAVNGIDVFRIFDAM
NDVRNLRVAIEAVKAAGKHAQGTIAYTTSPVHTVEAFVAQAKQLESMGCDSIAIKDMAGL
LTPYATGELVKALKAEQSLPIFIHSHDTAGLAAMCQLKAIENGADHIDTAISSFAWGTSH
PGTESMVAALKGSEFDTGLSLELLQEIGLYFYAVRKKYHQFESEFTAVDTRVQVNQVPGG
MISNLANQLKEQGALNRMNEVLAEIPRVREDLGFPPLVTPTSQIVGTQAFFNVLAGERYK
TITNEVKLYLQGGYGKAPGIVNEKLRRQAIGSEEVIDVRPADLLKPEMTKLRGEIGALAK
SEEDVLTYAMFPDIGRKFLEERDAGTLAPEVLLPIPEAGGVARVGGEGVPTEFVIDVHGE
SYRVDITGVGVKAEGKRHFYLSIDGMPEEVVFEPLNEFVSGGSSNRKHATAPGHVSTAMP
GNIVDVLVKEGDVVKAGQAVLITEAMKMETEVQAAIAGKVTAVHVAKGDRVNPGEILIEI
EG