Protein Info for PS417_28025 in Pseudomonas simiae WCS417

Annotation: 4-hydroxybenzoate polyprenyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 296 transmembrane" amino acids 29 to 46 (18 residues), see Phobius details amino acids 52 to 76 (25 residues), see Phobius details amino acids 102 to 119 (18 residues), see Phobius details amino acids 124 to 141 (18 residues), see Phobius details amino acids 146 to 164 (19 residues), see Phobius details amino acids 174 to 195 (22 residues), see Phobius details amino acids 216 to 237 (22 residues), see Phobius details amino acids 243 to 262 (20 residues), see Phobius details amino acids 274 to 295 (22 residues), see Phobius details TIGR01474: 4-hydroxybenzoate polyprenyl transferase" amino acids 18 to 293 (276 residues), 337.9 bits, see alignment E=2.7e-105 PF01040: UbiA" amino acids 34 to 279 (246 residues), 240.9 bits, see alignment E=6.9e-76

Best Hits

Swiss-Prot: 98% identical to UBIA_PSEFS: 4-hydroxybenzoate octaprenyltransferase (ubiA) from Pseudomonas fluorescens (strain SBW25)

KEGG orthology group: K03179, 4-hydroxybenzoate octaprenyltransferase [EC: 2.5.1.-] (inferred from 98% identity to pfs:PFLU6036)

MetaCyc: 77% identical to 4-hydroxybenzoate-polyprenyltransferase (Pseudomonas putida)
4-hydroxybenzoate nonaprenyltransferase. [EC: 2.5.1.39]

Predicted SEED Role

"4-hydroxybenzoate polyprenyltransferase (EC 2.5.1.39)" (EC 2.5.1.39)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.5.1.-

Use Curated BLAST to search for 2.5.1.- or 2.5.1.39

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7UU46 at UniProt or InterPro

Protein Sequence (296 amino acids)

>PS417_28025 4-hydroxybenzoate polyprenyltransferase (Pseudomonas simiae WCS417)
MYQRLLKSLNRLNPRAWDFIQLTRMDKPIGIYLLLWPTLWALWIAGKGSPSLINIVIFVL
GVVLTRAGGCVINDWADRKVDGHVKRTEQRPLVSGKISSKEALVFFAVLMGISFLLVLLT
NATTVLLSLGGLALAASYPFMKRYTYYPQVVLGAAFSWGMPMAFTAETGSLPAAAWLLYI
ANLLWTVGYDTYYAMTDRDDDLKIGVKSTAILFGEADRVIILTLQGLSLACLLLAGARFE
LGGWFHLGLLAAAGCFAWEFWYTRDKDRMKCFKAFLHNHWAGLAIFVGIVADYAFR