Protein Info for GFF5475 in Variovorax sp. SCN45

Annotation: Uncharacterized MFS-type transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 541 transmembrane" amino acids 34 to 57 (24 residues), see Phobius details amino acids 64 to 86 (23 residues), see Phobius details amino acids 97 to 118 (22 residues), see Phobius details amino acids 125 to 148 (24 residues), see Phobius details amino acids 180 to 207 (28 residues), see Phobius details amino acids 243 to 264 (22 residues), see Phobius details amino acids 273 to 294 (22 residues), see Phobius details amino acids 306 to 327 (22 residues), see Phobius details amino acids 334 to 356 (23 residues), see Phobius details amino acids 368 to 386 (19 residues), see Phobius details amino acids 392 to 414 (23 residues), see Phobius details PF05977: MFS_3" amino acids 23 to 538 (516 residues), 466.9 bits, see alignment E=7.8e-144 PF07690: MFS_1" amino acids 40 to 380 (341 residues), 118 bits, see alignment E=4.5e-38

Best Hits

KEGG orthology group: None (inferred from 93% identity to vpe:Varpa_5850)

Predicted SEED Role

"Probable major facilitator superfamily (MFS) transporter"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (541 amino acids)

>GFF5475 Uncharacterized MFS-type transporter (Variovorax sp. SCN45)
MPPISPEETPIPPQRPQPSKLAALEPLKLPVFRMLWSTWLIANICMWMNDVAAAWMMTSL
TTSPIWVALVQSASTLPVFLLGLPSGALADILDRRRWLVATQFWLAGTAVVLCAALALDL
MTAPLLLALTFANGIGLALRWPVFAAIVPELVPRPQLPAALGLNGIAMNASRIVGPLTAG
MLIASAGTVWVFALNAVLSVASGFVVLRWRREHTPNPLGREKLVSAMRVGVQFVRQSQRM
RAVLTRISIFFFHSTALLALLPLLARNLEGGGAGTFTLLLAAMGSGAIIAVLFLPRLRQA
LGRDQLVLRGTLLQSGATAVMAVAPNAWVAVPAMFFGGMAWITVANSLSVSAQLALPDWV
RARGMSMYQMAIMGASAIGAAIWGQVATIGTLQISLVIAAASGTLLMLAALRWVTDVSGE
DDTRPAQAGWAAGPPAEAPQEDGRVVITVEFLIDPARAAAFHLVMQQTRRARLSQGAIGW
ELLHDIAQPERYVEQIVDESWTDHLRRFNRATASDMALRERRLAFHLGESPPVVTRYVVR
R