Protein Info for PS417_27905 in Pseudomonas simiae WCS417

Annotation: serine/threonine protein kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 984 transmembrane" amino acids 319 to 341 (23 residues), see Phobius details PF00069: Pkinase" amino acids 8 to 250 (243 residues), 164.6 bits, see alignment E=6.3e-52 PF07714: PK_Tyr_Ser-Thr" amino acids 11 to 250 (240 residues), 118.6 bits, see alignment E=6e-38

Best Hits

KEGG orthology group: K11912, serine/threonine-protein kinase PpkA [EC: 2.7.11.1] (inferred from 95% identity to pfs:PFLU6010)

Predicted SEED Role

"Serine/threonine protein kinase (EC 2.7.11.1) PpkA" (EC 2.7.11.1)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.11.1

Use Curated BLAST to search for 2.7.11.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See U1UF34 at UniProt or InterPro

Protein Sequence (984 amino acids)

>PS417_27905 serine/threonine protein kinase (Pseudomonas simiae WCS417)
MNIVIPGYDIEGEIGEGAMASVYLATQRSLERKVALKVMAAALAADPSFCERFLREGKTL
ARLSHPHTVTIHDIGNVGELYYMAMEYLPNGTLKERIAAGMTPEQGVTLIRQIASALGYA
HAQGLVHRDVKPANILFRADGTAVLSDFGIAKSLDDRTQFTQAGFAVGTPSYMSPEQARG
QEIDGRADLYALGVVLYEILVGKLPYTGTDALSTALAHLTEPLPELPVHHGRYQDVLRKL
LAKDPAERFPDAAALLQALDNLPSESPEATLVRPLPIPMSFDLAGITPESIEIPTEKPQP
VRQPVVPPIQHSEQRRGPVLALAAVAVAVALAIGGASYWWLSGGDEPVAKPPAAAVPAVA
SPVAEADGGQRPLLMAGKKTLFQRVLSKPGAKLSADAGSAPGKALPAFSVLYVYQRKDVD
GSPWVRVGAATDGRSDGWLPASQVSDWKQSLVLKFTERSGRAPVMFLRQSAEVEKLLADP
AAAKGVLAKAQKNSEDNSQILALEPAASAVPQNQFYLLPIFDAKESFDENGQPVQLLNVA
SIDPGTSAAKPATPVISANADAFRTAVVLVVDTTVSMQPYIDQVRDVVHELQTRIGERGE
LDSVSFGMVGFRSSIKKTPGLEYVAKTLITLDQGRDPQRFLDMARQVKASTVSSHSFNED
AFAGVMQAVDGMDWSGYGGRIILLVTDAGALRKNDPFAATQMNEAEVRQAALGKQIKIYA
LHLRTDAGKKTHAGAESQYRILTADANPQIGDLYTAVPGGDVRKLGERVDEIGTVFANLV
HQVRSNTPQPVPLLNSAPSLADKSAAVGYAMHMDFLGRKTASQAPQLVSAWTADRDLTNP
ALPAFQVCVMLTKLQLNDLQQSLKLIVDAARKTQTSPKDFFQEIASASAYMSRDPQALRK
GGNLADGGILGEYLEGLPYRSKSLNMTQDLWLSLSVAEQEDFIDELDSKIRLYETFHNDL
ANWVRFGDAEPGDALYRVPLSTLP