Protein Info for PS417_27810 in Pseudomonas simiae WCS417

Annotation: ribonuclease PH

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 240 TIGR01966: ribonuclease PH" amino acids 3 to 238 (236 residues), 370 bits, see alignment E=1.9e-115 PF01138: RNase_PH" amino acids 11 to 141 (131 residues), 108.5 bits, see alignment E=3.3e-35 PF03725: RNase_PH_C" amino acids 160 to 226 (67 residues), 53.2 bits, see alignment E=2.3e-18

Best Hits

Swiss-Prot: 100% identical to RNPH_PSEFS: Ribonuclease PH (rph) from Pseudomonas fluorescens (strain SBW25)

KEGG orthology group: K00989, ribonuclease PH [EC: 2.7.7.56] (inferred from 100% identity to pfs:PFLU5991)

Predicted SEED Role

"Ribonuclease PH (EC 2.7.7.56)" in subsystem Heat shock dnaK gene cluster extended or tRNA processing (EC 2.7.7.56)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.56

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7UEC3 at UniProt or InterPro

Protein Sequence (240 amino acids)

>PS417_27810 ribonuclease PH (Pseudomonas simiae WCS417)
MKRPSGRAADQLRSIRITRNYTKHAEGSVLVEFGDTKVICTVSVENGVPRFLKGQGQGWL
TAEYGMLPRATGERNQREASRGKQGGRTLEIQRLIGRSLRAALDMSKLGDVTLYVDCDVI
QADGGTRTASITGAMVALVDALKVIKKRGGLKAGDPLKQMIAAVSVGMYQGEPVLDLDYL
EDSAAETDLNVVMTSTGGFIEVQGTAEGAPFQPADLNAMLALAQKGMTEIFELQNAALAD