Protein Info for PS417_27695 in Pseudomonas simiae WCS417

Annotation: alanine racemase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 357 TIGR00492: alanine racemase" amino acids 3 to 354 (352 residues), 373.1 bits, see alignment E=7e-116 PF01168: Ala_racemase_N" amino acids 8 to 218 (211 residues), 243.5 bits, see alignment E=2.2e-76 PF00842: Ala_racemase_C" amino acids 232 to 354 (123 residues), 141.5 bits, see alignment E=1.2e-45

Best Hits

Swiss-Prot: 87% identical to ALR_PSEF5: Alanine racemase (alr) from Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5)

KEGG orthology group: K01775, alanine racemase [EC: 5.1.1.1] (inferred from 94% identity to pfs:PFLU5966)

MetaCyc: 80% identical to alanine racemase (Pseudomonas putida)
Alanine racemase. [EC: 5.1.1.1, 5.1.1.10]

Predicted SEED Role

"Alanine racemase (EC 5.1.1.1)" in subsystem Alanine biosynthesis or Pyruvate Alanine Serine Interconversions (EC 5.1.1.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 5.1.1.1 or 5.1.1.10

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7UQN4 at UniProt or InterPro

Protein Sequence (357 amino acids)

>PS417_27695 alanine racemase (Pseudomonas simiae WCS417)
MRPARALIDLQALRHNYQLAREVTGAKALAVIKADAYGHGAVRVAQALQAEADGFAVACI
EEALELRAAGIRAPVLLLEGFFEADELPLIIEHDFWCVVHSLWQLEAIEQAKLSKPLTIW
LKLDSGMHRVGLHPKDYHEAYQRLLASGKVAKIVLMSHFARADELDCVRSDEQVAVFDAA
RQGLSAEVSLRNSPSVIGWPNVSSDWVRPGIMLYGATPFGEDQAIASRLQPVMTLESKVI
CVRELPAGEPVGYGAKFITPKPMRIGVVAMGYADGYPRHAPTGTPVLVAGQRSQLLGRVS
MDMLCIDLTDLPQAGLGSTVELWGKNILASDVAVAAETIPYQLFCNLRRVPRLYSEA