Protein Info for PS417_27585 in Pseudomonas simiae WCS417

Annotation: diaminopimelate epimerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00652: diaminopimelate epimerase
  • amino acids 4 to 274 (271 residues), 308.6 bits, E=1.9e-96
• PF01678: DAP_epimerase
  • amino acids 5 to 126 (122 residues), 106.2 bits, E=6e-35
  • amino acids 154 to 269 (116 residues), 116.8 bits, E=3.2e-38

Best Hits

Swiss-Prot: 94% identical to DAPF_PSEFL: Diaminopimelate epimerase (dapF) from Pseudomonas fluorescens

KEGG orthology group: K01778, diaminopimelate epimerase [EC: 5.1.1.7] (inferred from 98% identity to pfs:PFLU5946)

MetaCyc: 55% identical to diaminopimelate epimerase (Escherichia coli K-12 substr. MG1655)
Diaminopimelate epimerase. [EC: 5.1.1.7]

Predicted SEED Role

"Diaminopimelate epimerase (EC 5.1.1.7)" in subsystem Lysine Biosynthesis DAP Pathway (EC 5.1.1.7)

MetaCyc Pathways

• aspartate superpathway (22/25 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (16/18 steps found)
• L-lysine biosynthesis I (9/9 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis II (13/15 steps found)
• L-lysine biosynthesis VI (6/7 steps found)
• L-lysine biosynthesis II (6/9 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Lysine biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 5.1.1.7

Use Curated BLAST to search for 5.1.1.7

Sequence Analysis Tools

See A0A1N7UJJ3 at UniProt or InterPro

Protein Sequence (276 amino acids)

>PS417_27585 diaminopimelate epimerase (Pseudomonas simiae WCS417)
MLLRFTKMHGLGNDFMVLDLVSQHAHILPKHAKQWGDRHTGIGFDQLLIVEAPSNPEVDF
RYRIFNADGSEVEQCGNGARCFARFVLDKRLTAKRQIRVETKSGVIELDIRSDGQISVNM
GAPRLVPADIPFQAPEQAASYALDVDGKTVEIAAVSMGNPHAVLRVNDINNAPVHELGPK
IEHHPRFPARVNVGFLQVIDRTRAQLRVWERGAGETQACGTGACAAAVAAISQGWMDSPL
LIDLPGGRLSIEWAGPGHPVMMTGPASRVYEGQVRL