Protein Info for GFF5383 in Variovorax sp. SCN45

Annotation: DNA topoisomerase III, Burkholderia type (EC 5.99.1.2)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 987 TIGR01056: DNA topoisomerase III" amino acids 3 to 645 (643 residues), 542 bits, see alignment E=1.2e-166 PF01751: Toprim" amino acids 4 to 140 (137 residues), 33 bits, see alignment E=1.1e-11 PF01131: Topoisom_bac" amino acids 160 to 623 (464 residues), 361.8 bits, see alignment E=1e-111 PF13342: Toprim_Crpt" amino acids 683 to 745 (63 residues), 54.8 bits, see alignment 1.5e-18 amino acids 797 to 857 (61 residues), 55.8 bits, see alignment 7.3e-19 PF02201: SWIB" amino acids 914 to 984 (71 residues), 88.1 bits, see alignment 5.3e-29

Best Hits

KEGG orthology group: K03169, DNA topoisomerase III [EC: 5.99.1.2] (inferred from 78% identity to adn:Alide_4198)

Predicted SEED Role

"DNA topoisomerase III, Burkholderia type (EC 5.99.1.2)" in subsystem DNA topoisomerases, Type I, ATP-independent (EC 5.99.1.2)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.2

Use Curated BLAST to search for 5.99.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (987 amino acids)

>GFF5383 DNA topoisomerase III, Burkholderia type (EC 5.99.1.2) (Variovorax sp. SCN45)
MTKTLVIAEKPSVAQDIVRALTPVAGKFDKHDEHFENDSYVVTSAVGHLVEIQAPEEFDV
KRGKWSFANLPVIPPHFDLKPVDKTKTRLNAVVKQAKRKDVTQLINACDAGREGELIFRL
IEQYAGGKSGLNKPVKRLWLQSMTPQAIRDGFDALRTEKQMQGLADAARSRSEADWLVGI
NGTRAMTAFNSRDGGFFLTTVGRVQTPTLSVVVEREEQIRKFVSRDYWEIHGTFAAEAGE
YPGKWFNPDWKKANAPLLANGEPDPEQRADRVWNEQEARAIADAARGKPATVTEESKPTT
QASPMLFDLTSLQREANGRFGFSAKTTLALAQSLYERHKALTYPRTDSRALPEDYLPVVK
DTMGMLANSGMKHLAPFAKQAVDGNYVRPNKRIFDNAKVSDHFAIIPTLQAPSGLSEAEQ
KLYDFVVRRFLSVFFPSAEYQVTTRISTVEQGGKKYPFRTDGKVLVKPGWLAIYGKEAQD
DEKEDDKDGKRLVPVKPGEIVKTESADTKGLKTRPPARYSEATLLGAMEGAGKTIDDDEL
REAMQEKGLGTPATRAATIEGLITEKYMLREGRELIPTAKAFQLMTLLRGLGVEELSKAE
LTGEWEYKLAQMEKGALSRDAFMREIAQMTEHIVKKAKEYDRDTVPGDYATLQTPCPNCG
GIVRENYRRYACTGKSGTGEDACGFSFGKSPAGRTFEVAEAEALLRDKHIGPLEGFRSKA
GWPFTSEIVIKFDDEAKNWKLEFDFGDDKNADTGEIVDFSEQDTVGPCPVCGAPVFEHGS
NYVCEKSVPTNAQPTPTCTFKTGKIILQQPVERAQMEKLLATGKTDLLDKFVSMRTRRAF
KAFLTWNAEEGKVTFEFAPREGGSKFPPRKTFGKAAPAGKTAAAKKVAAKKTPAAKKAPA
AKKAAAPRKPGAGLKPSDSLAAVIGAEPVARTEVIKKLWDYIKANGLQDATNKRAINADA
KLKPVFGKDQVTMFELAGIVGKHLSAT