Protein Info for GFF5360 in Sphingobium sp. HT1-2

Annotation: Cytochrome d ubiquinol oxidase subunit I (EC 1.10.3.-)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 464 transmembrane" amino acids 18 to 40 (23 residues), see Phobius details amino acids 53 to 74 (22 residues), see Phobius details amino acids 94 to 117 (24 residues), see Phobius details amino acids 124 to 144 (21 residues), see Phobius details amino acids 185 to 207 (23 residues), see Phobius details amino acids 219 to 236 (18 residues), see Phobius details amino acids 319 to 343 (25 residues), see Phobius details amino acids 355 to 377 (23 residues), see Phobius details amino acids 405 to 428 (24 residues), see Phobius details PF01654: Cyt_bd_oxida_I" amino acids 6 to 434 (429 residues), 538.9 bits, see alignment E=3.4e-166

Best Hits

KEGG orthology group: K00425, cytochrome bd-I oxidase subunit I [EC: 1.10.3.-] (inferred from 79% identity to swi:Swit_0416)

MetaCyc: 61% identical to cyanide insensitive ubiquinol oxidase subunit I (Pseudomonas putida KT2440)
RXN-6883 [EC: 1.10.3.11]

Predicted SEED Role

"putative Cytochrome bd2, subunit I" in subsystem Terminal cytochrome d ubiquinol oxidases or Terminal cytochrome oxidases

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 1.10.3.-, 1.10.3.11

Use Curated BLAST to search for 1.10.3.- or 1.10.3.11

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (464 amino acids)

>GFF5360 Cytochrome d ubiquinol oxidase subunit I (EC 1.10.3.-) (Sphingobium sp. HT1-2)
MDAIILARAQFAFTVSFHFLFPAFSIGLASYLAVLEALWLKTGKGVYANLFRYWLKIFAV
VFAMGVVSGIVMSYQFGTNWSVFSDKAGPVIGPLMAYEVLTAFFLEAGFLGVMLFGINKV
GRKLHFVATLAVALGTFISAFWILSVNSWMQTPVGYEVGANGQFLPGPSWLTIIFNPSFP
YRLFHTVLAAYLTTAFIVGAVGGWHLLKDRTNPGARKMFSMAMWMAALVAPIQIFAGDMH
GLNTLKHQPVKVMAMEGHFQSHPHGAPLILFGIPNSAEQRVDYVVEIPKASSLILKHQLD
APLKGLDTVPQADRPPVGIVFWAFRVMVGIGFAMLGIGLLSLIARWRKALYDWSLLHRFA
VLMGPSGLIAVLAGWIVTEVGRQPFTIYGLLRTTQSASPLDAPAVASSLLAFVIVYFTVF
GMGIWYLLHLMKKPPEAHETPLGDAPIRSAGITPAPAIIEGASA