Protein Info for GFF5345 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: 5-Enolpyruvylshikimate-3-phosphate synthase (EC 2.5.1.19) / Cytidylate kinase (EC 2.7.4.25)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01356: 3-phosphoshikimate 1-carboxyvinyltransferase
  • amino acids 17 to 447 (431 residues), 422.5 bits, E=1.6e-130
• PF00275: EPSP_synthase
  • amino acids 17 to 444 (428 residues), 373.5 bits, E=1.2e-115
• TIGR00017: cytidylate kinase
  • amino acids 456 to 671 (216 residues), 200.1 bits, E=3.7e-63
• PF02224: Cytidylate_kin
  • amino acids 458 to 672 (215 residues), 204.6 bits, E=1.6e-64

Best Hits

KEGG orthology group: K00800, 3-phosphoshikimate 1-carboxyvinyltransferase [EC: 2.5.1.19] (inferred from 71% identity to aav:Aave_3284)

Predicted SEED Role

"5-Enolpyruvylshikimate-3-phosphate synthase (EC 2.5.1.19) / Cytidylate kinase (EC 2.7.4.25)" in subsystem Chorismate Synthesis or Common Pathway For Synthesis of Aromatic Compounds (DAHP synthase to chorismate) (EC 2.5.1.19, EC 2.7.4.25)

MetaCyc Pathways

• superpathway of aromatic amino acid biosynthesis (18/18 steps found)
• superpathway of L-tryptophan biosynthesis (13/13 steps found)
• superpathway of L-phenylalanine biosynthesis (10/10 steps found)
• superpathway of L-tyrosine biosynthesis (10/10 steps found)
• chorismate biosynthesis I (7/7 steps found)
• chorismate biosynthesis from 3-dehydroquinate (5/5 steps found)
• pyrimidine deoxyribonucleotide phosphorylation (4/4 steps found)
• superpathway of chorismate metabolism (43/59 steps found)
• CMP phosphorylation (2/2 steps found)
• superpathway of pyrimidine ribonucleosides salvage (7/10 steps found)
• chorismate biosynthesis II (archaea) (8/12 steps found)
• superpathway of pyrimidine deoxyribonucleoside salvage (5/9 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Phenylalanine, tyrosine and tryptophan biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.5.1.19 or 2.7.4.25

Protein Sequence (683 amino acids)

>GFF5345 5-Enolpyruvylshikimate-3-phosphate synthase (EC 2.5.1.19) / Cytidylate kinase (EC 2.7.4.25) (Hydrogenophaga sp. GW460-11-11-14-LB1)
MFAIHHLDIPPLASAGGTVRLPGSKSISNRVLLLAALSLGHTDIADLLDSDDTRVMLKAL
ADLGCRIEPQPDGALRVHGLGGALPVPQAKLFLGNAGTAMRPLTASLALLAARHGGEFEL
SGIARMHERPIGDLVDALRQLGCPVACLANEGYPPLRLGNGQTHALQLDQPIRVRGDVSS
QFLTALLLALPLVAQERDIVIDVVGELISRPYIEITLNLLERFGVAVRRDGWQRFTIPAG
SRYQSPGRIAVEGDASSASYFIALGALAPATPGADGITIEGVGLSSIQGDIRFVEAARLM
GADISGETNRLHIRRGAWPLKAVDLDCNHIPDAAMTLAAMALYADGTTTLRNIASWRVKE
TDRIAAMANECRKLGATVEEGEDFIRITPPARAQDWRAASIHTYDDHRVAMCFSLAAFNP
ANLPVRIEDPKCVAKTFPDYFETLFQVCTTPRERIPVICVDGPTASGKGTLSAEVAEQLG
YHLLDSGVLYRLVGLAAEKAGLSTAEPDLKDPAHARRLGELAAGLQVRFEGLRTLFEGQD
VTDALRTEAAGMAASRVSSVPAVREALLGLQHAFRQLPGLVADGRDMGTVIFPDAPLKVF
LTASAEQRAVRRHKQLISKGIAANIDSLLADLKARDLRDTSRTHAPLKPAEDALQLDNSG
MDIEQSVQQVLDWWQGRTAFSGN