Protein Info for GFF5338 in Sphingobium sp. HT1-2

Annotation: Quino(hemo)protein alcohol dehydrogenase, PQQ-dependent (EC 1.1.2.8)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 620 signal peptide" amino acids 1 to 25 (25 residues), see Phobius details TIGR03075: PQQ-dependent dehydrogenase, methanol/ethanol family" amino acids 39 to 590 (552 residues), 702.6 bits, see alignment E=1.6e-215 PF13360: PQQ_2" amino acids 100 to 279 (180 residues), 55.4 bits, see alignment E=1.1e-18 amino acids 504 to 572 (69 residues), 31.1 bits, see alignment E=3e-11 PF01011: PQQ" amino acids 105 to 137 (33 residues), 27.6 bits, see alignment (E = 2.6e-10) amino acids 536 to 572 (37 residues), 37.6 bits, see alignment 1.8e-13 PF13570: PQQ_3" amino acids 124 to 172 (49 residues), 19.3 bits, see alignment 1.9e-07

Best Hits

Swiss-Prot: 67% identical to BOH_THABB: 1-butanol dehydrogenase (quinone) (boh) from Thauera butanivorans (strain ATCC 43655 / DSM 2080/ JCM 20651 / NBRC 103042 / IAM 12574 / Bu B1211)

KEGG orthology group: K00114, alcohol dehydrogenase (cytochrome c) [EC: 1.1.2.8] (inferred from 70% identity to azl:AZL_b04560)

MetaCyc: 67% identical to 1-butanol dehydrogenase (Thauera butanivorans)
RXN-17641 [EC: 1.1.5.11]

Predicted SEED Role

"quinoprotein alcohol dehydrogenase" in subsystem Respiratory dehydrogenases 1

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 1.1.2.8

Use Curated BLAST to search for 1.1.2.8 or 1.1.5.11

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (620 amino acids)

>GFF5338 Quino(hemo)protein alcohol dehydrogenase, PQQ-dependent (EC 1.1.2.8) (Sphingobium sp. HT1-2)
MRGARGRLAAMTALATMLAGPALGQNGATTKHDFKLPGVSWDDILNDEQSNDNVLMYGLG
VRAQRFSTLDQINSDTAKKLVPKWVFSFGDERQRGQESQALFYDGVIYVTASYSRIFALD
AVTGKKIWSYTARLPDDIRPCCDVINRGAAIYGDKIFLGTLDASMIALDRKTGKVVWNKK
FQDHKAGYTMTGAPTIVKDQKTGRVMLIHGSSGDEFGVVGELYARDPDTGEEIWMRPMVE
GHQGRLNGKPSSFTGDPKAPSWPNDKDGKKVEAWNHGGGAPWQSATFDVKTNTIVIGTGN
PAPWNTWKRSPGDSLYTSGQVYIEPSTGEPVGFFQHTPNDAWDFSGNNPIVLFDLEKDGK
TIRAGAHADRNGYFFLTDLDKLTKRDGKISKQKGSALGIYPFVPNISWASKWDVANGRPI
ENPGQRPPPPAPGQDKGEVIKVTPNFLGGTNWMPMSYNPGTGLFYIPSNDWSEDYWTENV
TYNKGAAYLGQGFRIRKQFDDHVGVLRALDPKTGKIAWEAKEHLPLWGGNLTTKGNIVVQ
GTSDGYVKLFNARTGEELWKFQTGSGVVSVPITWEMDGKQYIGLASGYGGAVPLWGGDMA
DLTRSVSQGGSFWVFELPEG