Protein Info for GFF5332 in Variovorax sp. SCN45
Annotation: Glutamate synthase [NADPH] large chain (EC 1.4.1.13)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K00265, glutamate synthase (NADPH/NADH) large chain [EC: 1.4.1.13 1.4.1.14] (inferred from 88% identity to aaa:Acav_0979)Predicted SEED Role
"Glutamate synthase [NADPH] large chain (EC 1.4.1.13)" in subsystem Ammonia assimilation or Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 1.4.1.13)
MetaCyc Pathways
- L-glutamate and L-glutamine biosynthesis (7/7 steps found)
- L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
- ammonia assimilation cycle III (3/3 steps found)
- superpathway of ammonia assimilation (plants) (3/3 steps found)
- L-glutamate biosynthesis I (2/2 steps found)
- ammonia assimilation cycle I (2/2 steps found)
- L-glutamate biosynthesis IV (1/1 steps found)
- L-glutamine degradation I (1/1 steps found)
- L-glutamine degradation II (1/1 steps found)
- L-citrulline biosynthesis (6/8 steps found)
- glutaminyl-tRNAgln biosynthesis via transamidation (3/4 steps found)
- superpathway of L-citrulline metabolism (8/12 steps found)
KEGG Metabolic Maps
- Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
- Glutamate metabolism
- Nitrogen metabolism
Isozymes
Compare fitness of predicted isozymes for: 1.4.1.13, 1.4.1.14
Use Curated BLAST to search for 1.4.1.13 or 1.4.1.14
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (1585 amino acids)
>GFF5332 Glutamate synthase [NADPH] large chain (EC 1.4.1.13) (Variovorax sp. SCN45) MTTAAEIQHLQEHGLYSGADEHDACGVGFVAHIKGEKSHAIVLQGLKILENLDHRGAVGA DKLMGDGAGILIQLPDHLYREEMAKQGVTLPPPGEYGVGMIFLPKEHASREACEQEMERA IKAEGQVLLGWRDVPVNRDMPMSPTVREKEPLLRQVFIGRGNDVIVQDALERKLYVIRKT ASANIQRLKLKHSKEYYVPSMSSRTVVYKGLLLADQVGTYYLDLQDKRCVSALGLVHQRF STNTFPEWPLAHPYRYVAHNGEINTVKGNYNWMKAREGVMSSPVLGADLQKLYPISFAGQ SDTATFDNCLELLTMAGYPISQAVMMMIPEPWEQHATMDTRRRAFYEYHAAMLEPWDGPA SIVFTDGRQIGATLDRNGLRPSRYCVTDDDLVIMASESGVLPVPEQKIVRKWRLQPGKMF LIDLEQGRMIDDEEVKSNLANSKPYKQWIENLRIKLDSVKAEPVSAPLSQVALLDRQQAF GYTQEDIKFLMSPMAQAGEEGIGSMGNDSPLAVLSSKNKPLYNYFKQLFAQVTNPPIDPI REAIVMSLVSFIGPKPNLLDINQVNPPMRLEVSQPILDFADMAKLRDIGKYTQGKFKSYV LDITYPLAWGDEGVEAKLASLCAEAVDAIKGGHNILIISDRGVSPTQVAIPAVLALSAVH QYLVREGLRTTAGLVVETGSAREVHHFGVLAGYGAEAVHPYLAMETLTAMHADLPGDLSA DKAVYNYVKAIGKGLSKIMSKMGVSTYMSYCGAQLFEAIGLNSETVNKYFTGTASRVEGI GVFEIAEEAIRMHKAAFGDDPVLANMLDAGGEYAWRTRGEEHMWTPDAIAKLQHSTRANN WNTYKEYAQLINDQNRRHLTLRGLFEFKIDPAKAIPVDEVEAASEIVKRFATGAMSLGSI STEAHSVLAVAMNRIGGKSNTGEGGEDPARYRNELKGIPIKQGDTLKSVIGAANVEVDMP LRDGDSLRSRIKQVASGRFGVTAEYLHSADQIQIKMAQGAKPGEGGQLPGGKVTEYIGKQ RYAVPGVGLISPPPHHDIYSIEDLAQLIHDLKNTAPHASISVKLVSEIGVGTIAAGVAKC KSDHVVIAGHDGGTGASPWSSIKHAGSPWEIGLAETQQTLVLNRLRSRIRVQADGQMKTG RDVAIGALLGADEFGFATAPLVVEGCIMMRKCHLNTCPVGVATQDPILRKKFSGKPEHVV NYFFFVAEEVRQIMAQLGIRKFDDLIGRADLLDMRKGIEHWKASGLDFSRLFALPNVPAD VARFHVENQDHGLDKALDVKLIEKSRPAIDKGEKVQFIEVARNVNRSVGAMLSGALTKVH PQGLPDDSIRIQLEGTGGQSFGAFLARGITLYLIGDANDYTGKGLSGGRVVVRPSLDFRG EAVRNTIVGNTALYGATTGEAYLCGVAGERFAVRLSGATAVIEGTGDHGCEYMTGGTVAV LGKTGRNFAAGMSGGVAFVYDEDGQFASRCNLAMVSLDKVLTSAEQTASVHRKIWHGGET DEAQLKKLLEDHHRWTGSKRARELLDTWAVSRTKFVKVFPNEYKRALGELHDRKVELTSS GNNAHIGLEPHALTAPAPQTAPASV