Protein Info for GFF5325 in Variovorax sp. SCN45

Annotation: 3-dehydroquinate synthase (EC 4.2.3.4)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01357: 3-dehydroquinate synthase
  • amino acids 22 to 354 (333 residues), 379.7 bits, E=7.1e-118
• PF01761: DHQ_synthase
  • amino acids 77 to 188 (112 residues), 162.7 bits, E=2.3e-52
• PF24621: DHQS_C
  • amino acids 190 to 333 (144 residues), 171.5 bits, E=1.1e-54

Best Hits

Swiss-Prot: 69% identical to AROB_BURM1: 3-dehydroquinate synthase (aroB) from Burkholderia multivorans (strain ATCC 17616 / 249)

KEGG orthology group: K01735, 3-dehydroquinate synthase [EC: 4.2.3.4] (inferred from 94% identity to vpe:Varpa_1232)

Predicted SEED Role

"3-dehydroquinate synthase (EC 4.2.3.4)" in subsystem Chorismate Synthesis or Common Pathway For Synthesis of Aromatic Compounds (DAHP synthase to chorismate) or Type IV pilus (EC 4.2.3.4)

MetaCyc Pathways

• superpathway of aromatic amino acid biosynthesis (18/18 steps found)
• superpathway of L-tryptophan biosynthesis (13/13 steps found)
• superpathway of L-phenylalanine biosynthesis (10/10 steps found)
• superpathway of L-tyrosine biosynthesis (10/10 steps found)
• chorismate biosynthesis I (7/7 steps found)
• 3-dehydroquinate biosynthesis I (2/2 steps found)
• superpathway of chorismate metabolism (42/59 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Phenylalanine, tyrosine and tryptophan biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.2.3.4

Protein Sequence (369 amino acids)

>GFF5325 3-dehydroquinate synthase (EC 4.2.3.4) (Variovorax sp. SCN45)
MSLSVLSAPPERVDIQLGDRSYPILIGAGLLDDPASFAATPAAASALIVSNTTVAPLYAK
ALLAALAGRFRTVHLLELPDGEVHKNLQTLNLIFDDLLGHGSDRKTVLFALGGGVVGDMT
GFAAASYMRGVPFVQVPTTLLAQVDSSVGGKTAINHPLGKNMIGAFYQPQLVVCDLGTLQ
TLPPRELSAGLAEVIKYGPIYDMAFFDWIEANIEALTAREPAALAHAVKRSCEIKALVVG
QDERETGLRAILNFGHTFGHAIESGLGYGEWLHGEAVGCGMVMAAHLSQRLGGVDAAFVE
RLTRLIERAGLPTVGPALGAERYLELMRVDKKSEAGEIRFVVIDKPGSAVVRSAPDALVR
EVLAQCCEG