Protein Info for GFF5321 in Variovorax sp. SCN45

Annotation: Type IV pilus biogenesis protein PilO

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 225 transmembrane" amino acids 37 to 60 (24 residues), see Phobius details PF04350: PilO" amino acids 66 to 208 (143 residues), 171.2 bits, see alignment E=7.7e-55

Best Hits

KEGG orthology group: K02664, type IV pilus assembly protein PilO (inferred from 87% identity to vpe:Varpa_1228)

Predicted SEED Role

"Type IV pilus biogenesis protein PilO" in subsystem Type IV pilus

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (225 amino acids)

>GFF5321 Type IV pilus biogenesis protein PilO (Variovorax sp. SCN45)
MASKRSLPKVDVGVALQRFSEQFRGLNPNDPSVWPAVPRYALCLAVTAFVLVALWFVWLT
NSNDELEAERGKEVTLKADYQKKVAQAANLDLLKKQREQVQQYVTLLEKQLPSKAEMDAL
LSDINQAGLGRSLQFELFRPGQVVVKDYYAELPIAVRVTGKYHDMGAFVADVAGLSRIVT
LNGVTITPQKDKDTLTMDATARTFRYLDDDERAAQKRAAAPKAKK