Protein Info for GFF532 in Xanthobacter sp. DMC5

Annotation: 3-hydroxyacyl-[acyl-carrier-protein] dehydratase FabZ

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 77% identical to FABZ_AZOC5: 3-hydroxyacyl-[acyl-carrier-protein] dehydratase FabZ (fabZ) from Azorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / JCM 20966 / NBRC 14845 / NCIMB 13405 / ORS 571)

KEGG orthology group: K02372, 3R-hydroxymyristoyl ACP dehydrase [EC: 4.2.1.-] (inferred from 85% identity to xau:Xaut_4429)

MetaCyc: 42% identical to beta-hydroxyacyl-[acyl carrier protein] dehydratase (Bacillus subtilis subtilis 168)
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase. [EC: 4.2.1.59]

Predicted SEED Role

"3-hydroxyacyl-[acyl-carrier-protein] dehydratase, FabZ form (EC 4.2.1.59)" (EC 4.2.1.59)

MetaCyc Pathways

superpathway of fatty acids biosynthesis (E. coli) (49/53 steps found)
superpathway of fatty acid biosynthesis II (plant) (38/43 steps found)
palmitate biosynthesis II (type II fatty acid synthase) (29/31 steps found)
superpathway of unsaturated fatty acids biosynthesis (E. coli) (18/20 steps found)
superpathway of fatty acid biosynthesis I (E. coli) (15/16 steps found)
oleate biosynthesis IV (anaerobic) (13/14 steps found)
(5Z)-dodecenoate biosynthesis I (6/6 steps found)
palmitoleate biosynthesis I (from (5Z)-dodec-5-enoate) (8/9 steps found)
fatty acid elongation -- saturated (5/5 steps found)
gondoate biosynthesis (anaerobic) (4/4 steps found)
(5Z)-dodecenoate biosynthesis II (5/6 steps found)
stearate biosynthesis II (bacteria and plants) (5/6 steps found)
cis-vaccenate biosynthesis (4/5 steps found)
octanoyl-[acyl-carrier protein] biosynthesis (mitochondria, yeast) (9/12 steps found)
biotin biosynthesis I (11/15 steps found)
palmitate biosynthesis III (21/29 steps found)
8-amino-7-oxononanoate biosynthesis I (8/11 steps found)
stearate biosynthesis IV (4/6 steps found)
anteiso-branched-chain fatty acid biosynthesis (24/34 steps found)
even iso-branched-chain fatty acid biosynthesis (24/34 steps found)
odd iso-branched-chain fatty acid biosynthesis (24/34 steps found)
8-amino-7-oxononanoate biosynthesis IV (3/5 steps found)
tetradecanoate biosynthesis (mitochondria) (17/25 steps found)
2-allylmalonyl-CoA biosynthesis (2/8 steps found)
streptorubin B biosynthesis (20/34 steps found)
mycolate biosynthesis (26/205 steps found)
superpathway of mycolate biosynthesis (27/239 steps found)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.-, 4.2.1.59

Use Curated BLAST to search for 4.2.1.- or 4.2.1.59

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (168 amino acids)

>GFF532 3-hydroxyacyl-[acyl-carrier-protein] dehydratase FabZ (Xanthobacter sp. DMC5)
MNDTTRAAGEAGAAQAEAPKVLGSADIQRVLACLPHRYPILMVDRVEQIDGDLSCIGVKN
VTANEPHFLGHFPGNPVMPGVLIIEGMAQTAGVVCVLGKENKEKPSLVYFMTIDEAKFRR
PVVPGDVLEYHMTRISRRRNMWWYRGEAKVRGELVAEAKVGAMIVENS