Protein Info for PS417_27220 in Pseudomonas simiae WCS417

Annotation: type II secretion system protein E

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 559 PF05157: MshEN" amino acids 64 to 150 (87 residues), 53.4 bits, see alignment E=2.2e-18 PF00437: T2SSE" amino acids 180 to 544 (365 residues), 428.1 bits, see alignment E=2.4e-132

Best Hits

KEGG orthology group: K02454, general secretion pathway protein E (inferred from 93% identity to pfs:PFLU5867)

Predicted SEED Role

"Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7U329 at UniProt or InterPro

Protein Sequence (559 amino acids)

>PS417_27220 type II secretion system protein E (Pseudomonas simiae WCS417)
MPVDQRLELHQLLPALVEGRLITPEIAQRLSALPASNTLHPLESIAAQGLCLETLTEWLA
QRAGQPYLRIDPLKIDVATVVPLMSHAFAQRHAILAVAVDAQTVTVASAQPHVTSWEAGL
SQVLKRSIKRVVANPQDITRCIGEFYRLAKSVSGADQKVVAAGNVDLLNLGAREQEPDAN
DAHIVNIVDWLLQYAFAQRASDIHIEPCREQGRVRFRIDGLLHDVYQFPPQVTMAVVSRL
KSLGRMNVAEKRRPQDGRVKTKSPTGAEVELRLSTLPTAFGEKLVLRIFDPQVLLKGFDQ
LGLSADDQRRWQSMTRQTHGIILVTGPTGSGKTSTLYTTLRQLATREVNLCTVEDPIEMV
EPTFNQMQVQHNIDLTFASGIRALLRQDPDIIMIGEIRDQETAEMAIQAALTGHLVLSTL
HTNDAPSAISRLQELGIAHYLIKATLLGVMAQRLVRVLCPHCKRTLGDAHEAVGCGECRQ
SGYRGRAGVYEIMVLNEALKTLITPGADLHALRQKAIAQGMCSLRMAGVKKVEAGLTTMA
EVLRVTPGDSVTNPLFDKQ