Protein Info for GFF531 in Sphingobium sp. HT1-2

Annotation: Benzoate 1,2-dioxygenase alpha subunit (EC 1.14.12.10)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00355: Rieske
  • amino acids 49 to 132 (84 residues), 63.5 bits, E=1.4e-21
• PF00848: Ring_hydroxyl_A
  • amino acids 200 to 378 (179 residues), 60 bits, E=3.2e-20

Best Hits

Swiss-Prot: 59% identical to CBDA_BURCE: 2-halobenzoate 1,2-dioxygenase large subunit (cbdA) from Burkholderia cepacia

KEGG orthology group: K05549, benzoate 1,2-dioxygenase alpha subunit [EC: 1.14.12.10] (inferred from 80% identity to swi:Swit_2634)

MetaCyc: 59% identical to 2-halobenzoate 1,2-dioxygenase large subunit (Burkholderia cepacia 2CBS)

Predicted SEED Role

"Ring hydroxylating dioxygenase, alpha subunit (EC 1.14.12.13)" in subsystem Benzoate degradation (EC 1.14.12.13)

MetaCyc Pathways

• benzoate degradation I (aerobic) (2/2 steps found)
• 2-chlorobenzoate degradation (1/1 steps found)
• mandelate degradation to acetyl-CoA (11/18 steps found)
• meta cleavage pathway of aromatic compounds (5/10 steps found)
• toluene degradation IV (aerobic) (via catechol) (5/13 steps found)
• superpathway of aromatic compound degradation via 3-oxoadipate (17/35 steps found)
• superpathway of aerobic toluene degradation (10/30 steps found)
• superpathway of aromatic compound degradation via 2-hydroxypentadienoate (13/42 steps found)

KEGG Metabolic Maps

• Benzoate degradation via CoA ligation
• Benzoate degradation via hydroxylation
• Fluorobenzoate degradation

Isozymes

Compare fitness of predicted isozymes for: 1.14.12.10

Use Curated BLAST to search for 1.14.12.10 or 1.14.12.13

Protein Sequence (453 amino acids)

>GFF531 Benzoate 1,2-dioxygenase alpha subunit (EC 1.14.12.10) (Sphingobium sp. HT1-2)
MTSPMAFLRDRVETAVVDDPETGIFRCRRDVFTDPELFDLEMKYIFESNWVYLAHESQVE
KKNDYFTTWIGHTPVILTRAKDGGLNCVVNACAHRGAKLCRRKRGNQPLFVCPFHGWSFK
TDGKLLRAKDASTGAYPDSFDHEGSHDLTRVRVESYRGFIFGSLNHDVLPLEDHLGEAKV
IIDQMVDQAPEGLEVLTGNSTYTFHGNWKMQMENGCDGYHVSSVHENYQSTMGRRAEGGT
KAVDANGWSKAPASGVYGFEHGHILLWTQVLNPEVRPVWNYKDQLVERLGEDKARFILEQ
TRNLALYPNVFLMDQFSTQIRVVRPIDVHTTEVTIYCYAPKGESAEDRAHRIRQYEDFFN
VTGMGTPDDLEEFRSCQSAYEGAGELWNDLSRGATRWIAGPDSNATAMGMKPLLSSERSE
DEGLFVRQHEFWAQIMLDGMAREAGAPMMEAAE