Protein Info for GFF5307 in Sphingobium sp. HT1-2

Annotation: FIG00883918: hypothetical protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 434 transmembrane" amino acids 34 to 45 (12 residues), see Phobius details amino acids 58 to 80 (23 residues), see Phobius details amino acids 91 to 111 (21 residues), see Phobius details amino acids 115 to 137 (23 residues), see Phobius details amino acids 159 to 179 (21 residues), see Phobius details amino acids 191 to 210 (20 residues), see Phobius details amino acids 245 to 264 (20 residues), see Phobius details amino acids 283 to 302 (20 residues), see Phobius details amino acids 310 to 331 (22 residues), see Phobius details amino acids 337 to 359 (23 residues), see Phobius details amino acids 371 to 392 (22 residues), see Phobius details amino acids 403 to 421 (19 residues), see Phobius details PF00083: Sugar_tr" amino acids 24 to 225 (202 residues), 91.6 bits, see alignment E=5.3e-30 amino acids 235 to 427 (193 residues), 51.8 bits, see alignment E=6.4e-18 PF07690: MFS_1" amino acids 54 to 370 (317 residues), 88.5 bits, see alignment E=4.3e-29 amino acids 281 to 425 (145 residues), 33.5 bits, see alignment E=2.2e-12

Best Hits

Swiss-Prot: 32% identical to PROP_SALTY: Proline/betaine transporter (proP) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K03761, MFS transporter, MHS family, alpha-ketoglutarate permease (inferred from 89% identity to sjp:SJA_C1-16660)

MetaCyc: 32% identical to osmolyte:H+ symporter ProP (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-29; TRANS-RXN-29A

Predicted SEED Role

"FIG00883918: hypothetical protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (434 amino acids)

>GFF5307 FIG00883918: hypothetical protein (Sphingobium sp. HT1-2)
MTSHEGQGAAPDKRARLKAIIGGSTGNLVEWYDWYAYAAFTLYFAPHFFPSEDRTAQLLS
AAGIFAVGFLMRPIGAWLMGVYADRHGRKSGLTLSVSLMCAGSLLIAVTPGYEVIGVGAP
LLLVLARLMQGLSIGGEYGASATYLTEMAGKDRRGFYSSFQYVTLIAGQLVAICVLLLLQ
SILTEAQLDAWGWRIPFAIGGALAVIVFWLRRGLAETQSFEVAKAEGAPKSGFIELITNH
PRETLTVMLLTAGGTIAFYAYSIYMQKFLVNTAGFGRETASEINGITLFIFMLLQPLAGA
LSDRIGRKPLMIGFGVMGMLCTYPIFATLAVTRDPLVAGLLVMAGLVIVTGYTSINAVVK
AELFPAHIRALGVALPYALANTLFGGTAEFVALWFKQSGIEPAFYIYVSVMIAISLTVYI
RMRDTKAHSRILED