Protein Info for GFF5302 in Sphingobium sp. HT1-2

Annotation: DNA polymerase III delta prime subunit (EC 2.7.7.7)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 323 PF13177: DNA_pol3_delta2" amino acids 7 to 177 (171 residues), 127.6 bits, see alignment E=4.5e-41 PF00004: AAA" amino acids 28 to 172 (145 residues), 27.7 bits, see alignment E=3.4e-10

Best Hits

KEGG orthology group: K02341, DNA polymerase III subunit delta' [EC: 2.7.7.7] (inferred from 78% identity to sch:Sphch_0716)

Predicted SEED Role

"DNA polymerase III delta prime subunit (EC 2.7.7.7)" in subsystem DNA-replication (EC 2.7.7.7)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.7

Use Curated BLAST to search for 2.7.7.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (323 amino acids)

>GFF5302 DNA polymerase III delta prime subunit (EC 2.7.7.7) (Sphingobium sp. HT1-2)
MTSLVGHDAQAQALLAAASSGRMHHGWILSGPRGIGKASFARAMAMRLLADAAGPKIEGE
GLAVPESHPIRRLIEASAHPDYAELAPLEKDGVTARNISVDQVRGLQRLIQSAPSLSSRR
IVVIDSADDLERGAANALLKTLEEPPADMIFLLVSHAPGRLLPTIRSRCRTMRFDPLSDG
AMATVLRREDESLSGPELDALLRAGEGSPGRALRYAGLDLAGLEKSLLAIAADGDPGNRE
RLRLAKTLAGKAAKPRYEAFLERAPAFIAQAARTRHGPALGSALDHWEAARHLAGGAVIL
SLDPASVVFELGGHVAALAEAAT