Protein Info for GFF5293 in Variovorax sp. SCN45

Annotation: N-carbamoyl-L-amino acid hydrolase (EC 3.5.1.87)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 416 TIGR01879: amidase, hydantoinase/carbamoylase family" amino acids 10 to 410 (401 residues), 392 bits, see alignment E=1.5e-121 PF01546: Peptidase_M20" amino acids 81 to 411 (331 residues), 80.9 bits, see alignment E=1.3e-26 PF07687: M20_dimer" amino acids 215 to 316 (102 residues), 26.1 bits, see alignment E=7.2e-10

Best Hits

KEGG orthology group: K06016, N-carbamoyl-L-amino-acid hydrolase [EC: 3.5.1.87] (inferred from 86% identity to vpe:Varpa_1203)

Predicted SEED Role

"N-carbamoyl-L-amino acid hydrolase (EC 3.5.1.87)" in subsystem Hydantoin metabolism (EC 3.5.1.87)

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.87

Use Curated BLAST to search for 3.5.1.87

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (416 amino acids)

>GFF5293 N-carbamoyl-L-amino acid hydrolase (EC 3.5.1.87) (Variovorax sp. SCN45)
MSPSISIQADRLWHRLETLSSFTLPDVPWTRRAFSPEFDKARQWLREQFEQAGLAVHTDA
AGNLTGRREGSEAGLAPLVTGSHCDTVVGGGRFDGILGVLAGLEVAQSLHEGGVVLRHPL
EVIDFLSEEPSDYGISCVGSRAFAGTLDAAMLASCNEAGESLSQAMQRIGARPDALAGAA
RGAGGVAAFVELHIEQGPVLEREGLPIGVVTDIVGIRRVALTVTGQPDHAGTTPMDIRRD
ALVGAARLIDTAHREASAMDGQPHYVVATIGRISMSPNVPNAVPGRVDMVLEVRSNAQSV
LDEFPERLLALCRDELQALRVSCAMEPLTRTPPVECSDVVMQAVAEAGDRLGLAHRRLPS
GAGHDAMHVASTGPMGMVFIPCLDGRSHCPEEWATKEQVAAGTLVLAETLRALDRG