Protein Info for GFF5277 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: Ribosomal small subunit pseudouridine synthase A (EC 4.2.1.70)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00849: PseudoU_synth_2
  • amino acids 73 to 210 (138 residues), 59.7 bits, E=2e-20
• TIGR00093: pseudouridine synthase
  • amino acids 77 to 241 (165 residues), 130.6 bits, E=2.3e-42

Best Hits

KEGG orthology group: K06183, ribosomal small subunit pseudouridine synthase A [EC: 5.4.99.12] (inferred from 73% identity to pna:Pnap_2786)

Predicted SEED Role

"Ribosomal small subunit pseudouridine synthase A (EC 4.2.1.70)" (EC 4.2.1.70)

MetaCyc Pathways

• pseudouridine degradation (1/2 steps found)

KEGG Metabolic Maps

• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.70, 5.4.99.12

Use Curated BLAST to search for 4.2.1.70 or 5.4.99.12

Protein Sequence (257 amino acids)

>GFF5277 Ribosomal small subunit pseudouridine synthase A (EC 4.2.1.70) (Hydrogenophaga sp. GW460-11-11-14-LB1)
VSGDNSHTMMQLQDILFSQGFGTRRICSGLVQQGLVSVGEDTQPCTDPSALFEEEGLVFR
VQGELWHYHARAYLLLNKPAGYECSQKPSTYPSVYTLLPGPLRQRGGGAAAGVQAVGRLD
QDTTGLLLLSDDGKFIHRMTSPRHHVPKVYEAQVKHPLDARQVSRLLDGVVLDDDPQPVK
AQACEAVSELHLRLTLTEGKYHQVKRMVAAVGNRVEGLHRSSIGGLVLSPELKPGQWRWL
TAAEVLALTQKQAAAGA