Protein Info for GFF5273 in Sphingobium sp. HT1-2

Annotation: Alkaline phosphatase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 543 signal peptide" amino acids 1 to 31 (31 residues), see Phobius details PF16655: PhoD_N" amino acids 41 to 131 (91 residues), 91.9 bits, see alignment E=3.2e-30 PF09423: PhoD" amino acids 142 to 513 (372 residues), 295.5 bits, see alignment E=5.6e-92

Best Hits

KEGG orthology group: K01113, alkaline phosphatase D [EC: 3.1.3.1] (inferred from 67% identity to psu:Psesu_1601)

Predicted SEED Role

"Alkaline phosphatase"

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.3.1

Use Curated BLAST to search for 3.1.3.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (543 amino acids)

>GFF5273 Alkaline phosphatase (Sphingobium sp. HT1-2)
MVMQIDRRCMILGGVWGIGALALPAGRALAADLIGARGFTHAVASGEPGADSMLLWTRYV
PASEVESARLDVEVAIDPDFVRIVGGGTVRTGGYRDWTAKVTVDGLQPGTSYWYRFVAPD
GSKSPVGRTRTLPQGDVPRFGLGVFSCSNLPYGWFNAYAHAAARDDLDLWLHVGDYIYEY
GNASVRPGQGIVGRTLMPDTEILAIADYRLRYATYRADPDLQRLHQMAPMVALWDDHESA
NDSWEGGAQNHQADKEGDWNTRRAAAMQVYREWMPVSDEPWKAYDIGTLATLYRTESRLL
ARTRPADITAAASAPDADAALKAFHDGVWQDPSATMLGSTQESWLAHAFRANARTTAWQM
VGMGTIIGRTVMPANVTDWLRPDANAKLVQRYRNSVRMAQNGLPMWMDRWDGYPAARSRL
LRSAQAADSDLIMLAGDSHNAWAYGLVEDGQPAGVEFAGQAVTSGGMEGDLRGDPTAVAR
GFVAANRELKWANTHQRGYMMIDISRDRVTGEWLFLDTIKARSTRIASTHRMSVERGRRV
FSA