Protein Info for PS417_26955 in Pseudomonas simiae WCS417

Annotation: ATPase P

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 748 transmembrane" amino acids 134 to 153 (20 residues), see Phobius details amino acids 159 to 177 (19 residues), see Phobius details amino acids 189 to 205 (17 residues), see Phobius details amino acids 211 to 229 (19 residues), see Phobius details amino acids 363 to 387 (25 residues), see Phobius details amino acids 393 to 419 (27 residues), see Phobius details amino acids 702 to 720 (19 residues), see Phobius details amino acids 725 to 746 (22 residues), see Phobius details TIGR01511: copper-translocating P-type ATPase" amino acids 173 to 745 (573 residues), 398.8 bits, see alignment E=9.2e-123 TIGR01525: heavy metal translocating P-type ATPase" amino acids 191 to 745 (555 residues), 584.2 bits, see alignment E=6.9e-179 TIGR01494: HAD ATPase, P-type, family IC" amino acids 213 to 728 (516 residues), 264.4 bits, see alignment E=3.1e-82 PF00122: E1-E2_ATPase" amino acids 242 to 423 (182 residues), 179.4 bits, see alignment E=7.8e-57 PF00702: Hydrolase" amino acids 442 to 655 (214 residues), 124.1 bits, see alignment E=1.4e-39

Best Hits

KEGG orthology group: K01534, Cd2+/Zn2+-exporting ATPase [EC: 3.6.3.3 3.6.3.5] (inferred from 97% identity to pfs:PFLU5813)

MetaCyc: 76% identical to cadmium translocating P-type ATPase (Pseudomonas putida KT2440)
3.6.3.3-RXN [EC: 7.2.2.21]

Predicted SEED Role

"Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4)" (EC 3.6.3.3, EC 3.6.3.4, EC 3.6.3.5)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.3, 3.6.3.4, 3.6.3.5

Use Curated BLAST to search for 3.6.3.3 or 3.6.3.4 or 3.6.3.5 or 7.2.2.21

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7U897 at UniProt or InterPro

Protein Sequence (748 amino acids)

>PS417_26955 ATPase P (Pseudomonas simiae WCS417)
MSDSIHTPHKHDHDHGPKKLTPVHDHGHGGSCCSGTPAPALVQLSEAPTAGSRLSTFRIE
AMDCPTEQTLIQNKLGKLAGVQQLEFNLINRILGVTHDLPSTAPIIDAIKSLGMQADPIE
EGTPAAEPPAKKHWWPLALSGVGALGAEVLHFANAAPTWVIALVALVSILSGGLTTYKKG
WIALKNLNLNINALMSIAVTGAILIGQWPEAAMVMFLFTVAELIEAKSLDRARNAISGLM
QMTPEQATVQQSDGSWAEQEVKSIELGAIVRVKPGERIGLDGEVTAGQSTIDQAPITGES
LPIEKTVGDKVFAGTINQAGSLEYTVTAAANNSTLARIIHAVEQAQGARAPTQRFVDSFS
KIYTPAVFLFALGVALIPPLFMAGVWFDWVYRALVLLVVACPCALVISTPVTIVSGLAAA
ARKGILIKGGVYLEGGYKLDYLALDKTGTITHGKPVQTDYLALFPNVADSAPALAASLAG
RSDHPVSLAIANAAVDKNLPSHAVDNFEALAGRGVRGDINGETYHLGNHRLVEDLGLCSP
ELEEKLFALEKQGKSVVLLLDKSGPLALFAVADTVKDSSREAIQQLHDLGIKTLMLTGDN
THTAQAIAAQVGIDQAQGDLLPTDKLQAIETLYGQGHRVGMVGDGINDAPALARAEIGFA
MAAAGTDTAIETADVALMDDDLRKIPAFIRLSRQTSSILKQNIALALVIKAIFLAVTFLG
MATMWMAVFADMGVSLLVVFNGLRLLRK