Protein Info for GFF5264 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: Dihydrofolate reductase (EC 1.5.1.3)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00186: DHFR_1
  • amino acids 8 to 165 (158 residues), 180.3 bits, E=1.1e-57

Best Hits

Swiss-Prot: 49% identical to DYR3_SALTM: Dihydrofolate reductase type 3 (dhfrIII) from Salmonella typhimurium

KEGG orthology group: None (inferred from 73% identity to hmg:100209486)

Predicted SEED Role

"Dihydrofolate reductase (EC 1.5.1.3)" in subsystem Folate Biosynthesis (EC 1.5.1.3)

MetaCyc Pathways

• superpathway of tetrahydrofolate biosynthesis and salvage (11/12 steps found)
• superpathway of tetrahydrofolate biosynthesis (9/10 steps found)
• dTMP de novo biosynthesis (mitochondrial) (3/3 steps found)
• tetrahydrofolate biosynthesis I (3/3 steps found)
• superpathway of chorismate metabolism (43/59 steps found)
• folate transformations III (E. coli) (7/9 steps found)
• folate transformations II (plants) (8/11 steps found)

KEGG Metabolic Maps

• Biosynthesis of phenylpropanoids
• Folate biosynthesis
• One carbon pool by folate

Isozymes

Compare fitness of predicted isozymes for: 1.5.1.3

Use Curated BLAST to search for 1.5.1.3

Protein Sequence (169 amino acids)

>GFF5264 Dihydrofolate reductase (EC 1.5.1.3) (Hydrogenophaga sp. GW460-11-11-14-LB1)
MPQPTTRLHLILARAANGVIGKDNALPWHLPEDMAHFKRTTLGHPVIMGRKTWDSLPPKF
RPLPGRLNIVVTRDTAWQAEGASRANSLPQAVSLCPAGSDAWVIGGAQIYAAALPLADTA
VVTEIARDFEGDAHAPVFGPEWQETARETHTAANGLPFAFVTYTRTTTR